AJP - Heart BIOPAC complete lab solutions
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 277: H940-H946, 1999;
0363-6135/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow A corrigendum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Protas, L.
Right arrow Articles by Robinson, R. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Protas, L.
Right arrow Articles by Robinson, R. B.
Vol. 277, Issue 3, H940-H946, September 1999

Neuropeptide Y contributes to innervation-dependent increase in ICa,L via ventricular Y2 receptors

Lev Protas and Richard B. Robinson

Department of Pharmacology, Columbia University, New York, New York 10032

The developmental increase in L-type Ca current (ICa,L) density in the rat ventricle is reproduced in vitro by culturing neonatal myocytes with sympathetic neurons. We tested whether this effect of sympathetic innervation results from a chronic or sustained action of neurally released neuropeptide Y (NPY). Ventricular myocytes from newborn rats were cultured in serum-free medium with or without sympathetic neurons, NPY, or NPY analogs. Ca currents were measured in single myocytes at room temperature using the perforated patch clamp. In all cell groups (control, innervated, or NPY treated), the current-voltage relation for ICa,L was represented by a bell-shaped curve with maximal value near 0 mV. The current density at 0 mV normalized to that of corresponding mean control values was 1.63 ± 0.12 and 1.52 ± 0.16 for innervated and NPY-treated myocytes, respectively. Both groups differed significantly from control (P < 0.05). NPY analogs exhibited the following rank order of effectiveness: NPY >=  NPY-(13---36) >=  PYY >> [Leu31Pro34]NPY, suggesting that the NPY effect occurs via a Y2-receptor subtype. In confirmation, chronic treatment of innervated cultures with a Y2-selective NPY antagonist prevented the innervation-dependent increase in ICa,L. These results indicate that sympathetic innervation contributes to the developmental increase in ICa,L via neurally released NPY acting at Y2 receptors on the ventricular myocytes.

sympathetic innervation; neonatal cardiomyocytes; calcium current


This article has been cited by other articles:


Home page
 J. Physiol.Home page
X. Zhao, A. Bucchi, R. V. Oren, Y. Kryukova, W. Dun, C. E. Clancy, and R. B. Robinson
In vitro characterization of HCN channel kinetics and frequency dependence in myocytes predicts biological pacemaker functionality
J. Physiol., April 1, 2009; 587(7): 1513 - 1525.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
L. Protas, J. Qu, and R. B. Robinson
Neuropeptide Y: Neurotransmitter or Trophic Factor in the Heart?
Physiology, October 1, 2003; 18(5): 181 - 185.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
J. Qu, A. Barbuti, L. Protas, B. Santoro, I. S. Cohen, and R. B. Robinson
HCN2 Overexpression in Newborn and Adult Ventricular Myocytes : Distinct Effects on Gating and Excitability
Circ. Res., July 6, 2001; 89 (1): e8 - e14.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
G. Bru-Mercier, E. Deroubaix, D. Rousseau, A. Coulombe, and J.-F. Renaud
Depressed transient outward potassium current density in catecholamine-depleted rat ventricular myocytes
Am J Physiol Heart Circ Physiol, April 1, 2002; 282(4): H1237 - H1247.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online