AJP - Heart Calcium Transients and Cell-Sarcomere
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 277: H1521-H1531, 1999;
0363-6135/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Coceani, F.
Right arrow Articles by Yanagisawa, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Coceani, F.
Right arrow Articles by Yanagisawa, M.
Vol. 277, Issue 4, H1521-H1531, October 1999

Endothelin A receptor is necessary for O2 constriction but not closure of ductus arteriosus

F. Coceani1, Y.-A. Liu1, E. Seidlitz1, L. Kelsey1, T. Kuwaki3, C. Ackerley2, and M. Yanagisawa4

1 Integrative Biology Programme and 2 Division of Pathology, The Hospital for Sick Children, Toronto, Ontario, Canada M5G 1X8; 3 Department of Physiology, School of Medicine, Chiba University, Chiba, 260-8670 Japan; and 4 Howard Hughes Medical Institute and Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75235

In vitro and in vivo techniques were developed with genetically modified mice to determine whether endothelin-1 (ET-1) functions as an O2 mediator in closure of the ductus arteriosus (DA) at birth. Wild-type CD-1 and 129/SvEv mice with ETA receptor -/-, +/-, and +/+ genotypes were used. Isolated DA from term ETA +/+ fetuses contracted to O2 (5-95%) and a thromboxane A2 analog (ONO-11113, 0.1 µM). Instead, ET-1 elicited a dual response with weak relaxation (0.1 nM) preceding contraction (1-100 nM). Indomethacin (2.8 µM) was also a constrictor. ETA -/- DA, unlike ETA +/+ DA, contracted marginally to O2 and ET-1 but responded to ONO-11113. O2 contraction was also reduced in ETA +/- DA. In vivo, DA constricted equally in tracheotomized ETA -/- and ETA +/+ newborns. Conversely, no DA constriction was seen in hyperoxic ETA -/- fetuses in utero, although it occurred in ETA +/+ and +/- littermates. We conclude that ET-1 mediates the DA constrictor response to O2. Without ET-1, however, the vessel still closes postnatally, conceivably caused by the withdrawal of relaxing influence(s).

oxygen; endothelin


This article has been cited by other articles:


Home page
 Physiol. GenomicsHome page
M. Costa, S. Barogi, N. D. Socci, D. Angeloni, M. Maffei, B. Baragatti, C. Chiellini, E. Grasso, and F. Coceani
Gene expression in ductus arteriosus and aorta: comparison of birth and oxygen effects
Physiol Genomics, April 13, 2006; 25(2): 250 - 262.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
Y. Coe, S. J. Haleen, K. M. Welch, Y.-A. Liu, and F. Coceani
The Endothelin A Receptor Antagonists PD 156707 (CI-1020) and PD 180988 (CI-1034) Reverse the Hypoxic Pulmonary Vasoconstriction in the Perinatal Lamb
J. Pharmacol. Exp. Ther., August 1, 2002; 302(2): 672 - 680.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online