AJP - Heart Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 277: H1641-H1646, 1999;
0363-6135/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kan, H.
Right arrow Articles by Finkel, M. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kan, H.
Right arrow Articles by Finkel, M. S.
Vol. 277, Issue 4, H1641-H1646, October 1999

RAPID COMMUNICATION
TNF-alpha enhances cardiac myocyte NO production through MAP kinase-mediated NF-kappa B activation

Hong Kan1, Zirong Xie1, and Mitchell S. Finkel1,2,3

Departments of 1 Medicine and 2 Pharmacology, West Virginia University School of Medicine, Robert C. Byrd Health Sciences Center, Morgantown 26506-9157; and 3 Louis A. Johnson Veterans Affairs Medical Center, Clarksburg, West Virginia 26301

We have previously reported that interleukin-1beta (IL-1beta ) alone induced nitric oxide (NO) production by neonatal rat cardiac myocytes (CM). The effects of tumor necrosis factor-alpha (TNF-alpha ) on inducible NO synthase (iNOS) were not characterized. Unlike IL-1beta , TNF-alpha alone failed to enhance NO production in CM. However, the addition of TNF-alpha to IL-1beta significantly enhanced iNOS mRNA expression, iNOS protein synthesis, and NO production (NO-2). TNF-alpha enhancement of IL-1beta -induced NO-2 production was blocked by PD-98059, a selective mitogen-activated protein (MAP) kinase kinase inhibitor, but not calphostin C (Cal C), a protein kinase C inhibitor. TNF-alpha -enhanced MAP kinase activity was associated with an increase in IL-1beta -stimulated NF-kappa B activity. PD-98059, but not Cal C, inhibited both TNF-alpha -enhanced MAP kinase and NF-kappa B activities. Thus TNF-alpha enhancement of IL-1beta -induced NO production is associated with MAP kinase-mediated activation of NF-kappa B.

cytokines; heart; cell signaling; tumor necrosis factor; nitric oxide; mitogen-activated protein kinase


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. Zhang, G. Gong, Y. Ye, T. Guo, A. Mansoor, Q. Hu, K. Ochiai, J. Liu, X. Wang, Y. Cheng, et al.
Nitric oxide regulation of myocardial O2 consumption and HEP metabolism
Am J Physiol Heart Circ Physiol, January 1, 2005; 288(1): H310 - H316.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. R. Morris, R. D. Lutz, H.-S. Choi, T. Kamitani, K. Chmura, and E. D. Chan
Role of the NF-{kappa}B Signaling Pathway and {kappa}B cis-Regulatory Elements on the IRF-1 and iNOS Promoter Regions in Mycobacterial Lipoarabinomannan Induction of Nitric Oxide
Infect. Immun., March 1, 2003; 71(3): 1442 - 1452.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
D. B. Sawyer and J. Loscalzo
Myocardial Hibernation: Restorative or Preterminal Sleep?
Circulation, April 2, 2002; 105(13): 1517 - 1519.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
H. Kan, Z. Xie, and M. S. Finkel
HIV gp120 enhances NO production by cardiac myocytes through p38 MAP kinase-mediated NF-kappa B activation
Am J Physiol Heart Circ Physiol, December 1, 2000; 279(6): H3138 - H3143.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online