AJP - Heart Calcium Transients and Cell-Sarcomere
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Am J Physiol Heart Circ Physiol 277: H1841-H1848, 1999;
0363-6135/99 $5.00
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Vol. 277, Issue 5, H1841-H1848, November 1999

Gs and adenylyl cyclase in transverse tubules of heart: implications for cAMP-dependent signaling

Michael A. Laflamme and Peter L. Becker

Department of Physiology, Emory University School of Medicine, Atlanta, Georgia 30322

The transverse tubules are highly specialized invaginations of the cardiac sarcolemmal membrane involved in excitation-contraction (EC) coupling. Several proteins directly involved in EC coupling have been shown to reside either in the transverse tubular membrane or in closely associated structures. With the use of immunofluorescence microscopy, we have found that GS and adenylyl cyclase, key elements in the beta -adrenergic signal transduction cascade, are essentially homogeneously distributed throughout the transverse tubular network of isolated rat ventricular myocytes. GS, in particular, was much more abundant within the transverse tubular membrane than in the peripheral sarcolemma. Furthermore, both proteins are also present in the intercalated disk region. The location of these elements of the cAMP-signaling cascade within a few micrometers of every inotropic target suggests that control and action of this second messenger are quite local. Furthermore, a similar distribution is likely for negatively inotropic receptor systems that oppose GS-linked receptors at the level of adenylyl cyclase. Thus, in addition to their role in EC coupling, transverse tubules appear to be the primary site for signaling by inotropic agents.

signal transduction; immunolocalization


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