| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Medical Physics, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands; and 2 Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia 22906-0011
We
previously reported that a 0.4- to 0.5-µm-thick endothelial surface
layer confines Dextran 70 (70 kDa) to the central core of hamster
cremaster muscle capillaries. In the present study we used a variety of
plasma tracers to probe the barrier properties of the endothelial
surface layer using combined fluorescence and brightfield intravital
microscopy. No permeation of the endothelial surface layer was observed
for either neutral or anionic dextrans 
70 kDa, but a neutral Dextran
40 (40 kDa) and neutral free dye (rhodamine, 0.4 kDa)
equilibrated with the endothelial surface layer within 1 min. In
contrast, small anionic tracers of similar size (0.4-40 kDa)
permeated the endothelial surface layer relatively slowly with
half-times (
50) between 11 and 60 min, depending on
tracer size. Furthermore, two plasma proteins, fibrinogen (340 kDa) and
albumin (67 kDa), moved slowly into the endothelial surface layer at
the same rates, despite greatly differing sizes (50 
40 min). Dextran 70, which did not enter the glycocalyx over the course
of these experiments, entered at the same rate as free albumin when it
was conjugated to albumin. These findings
demonstrate that for anionic molecules size and charge have a profound
effect on the penetration rate into the glycocalyx. The equal rates of penetration of the glycocalyx demonstrated by the different
protein molecules suggests that multiple factors may influence the
penetration of the barrier, including molecular size, charge, and structure.
intravital microscopy; capillaries; endothelium; glycocalyx; solute barrier
This article has been cited by other articles:
|
|
 |
|
  B. Rippe Free water transport, small pore transport and the osmotic pressure gradient three-pore model of peritoneal transport Nephrol. Dial. Transplant., July 1, 2008; 23(7): 2147 - 2153. [Full Text] [PDF]
|
|
|
|
|
|
M. F. Flessner Distributed model of peritoneal transport: implications of the endothelial glycocalyx Nephrol. Dial. Transplant., July 1, 2008; 23(7): 2142 - 2146. [Full Text] [PDF]
|
|
|
|
 |
|
 J. W. G. E. VanTeeffelen, A. A. Constantinescu, J. Brands, J. A. E. Spaan, and H. Vink Bradykinin- and sodium nitroprusside-induced increases in capillary tube haematocrit in mouse cremaster muscle are associated with impaired glycocalyx barrier properties J. Physiol., July 1, 2008; 586(13): 3207 - 3218. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Haraldsson, J. Nystrom, and W. M. Deen Properties of the Glomerular Barrier and Mechanisms of Proteinuria Physiol Rev, April 1, 2008; 88(2): 451 - 487. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Nieuwdorp, M. C. Meuwese, H. L. Mooij, C. Ince, L. N. Broekhuizen, J. J. P. Kastelein, E. S. G. Stroes, and H. Vink Measuring endothelial glycocalyx dimensions in humans: a potential novel tool to monitor vascular vulnerability J Appl Physiol, March 1, 2008; 104(3): 845 - 852. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. H. J. Salmon, I. Toma, A. Sipos, P. R. Muston, S. J. Harper, D. O. Bates, C. R. Neal, and J. Peti-Peterdi Evidence for restriction of fluid and solute movement across the glomerular capillary wall by the subpodocyte space Am J Physiol Renal Physiol, December 1, 2007; 293(6): F1777 - F1786. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. P. Stevens, V. Hlady, and R. O. Dull Fluorescence correlation spectroscopy can probe albumin dynamics inside lung endothelial glycocalyx Am J Physiol Lung Cell Mol Physiol, August 1, 2007; 293(2): L328 - L335. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Yao, A. Rabodzey, and C. F. Dewey Jr. Glycocalyx modulates the motility and proliferative response of vascular endothelium to fluid shear stress Am J Physiol Heart Circ Physiol, August 1, 2007; 293(2): H1023 - H1030. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. W.G.E. VanTeeffelen, J. Brands, C. Jansen, J. A.E. Spaan, and H. Vink Heparin Impairs Glycocalyx Barrier Properties and Attenuates Shear Dependent Vasodilation in Mice Hypertension, July 1, 2007; 50(1): 261 - 267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Cabrales, B. Y. S. Vazquez, A. G. Tsai, and M. Intaglietta Microvascular and capillary perfusion following glycocalyx degradation J Appl Physiol, June 1, 2007; 102(6): 2251 - 2259. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Jacob, P. Conzen, U. Finsterer, A. Krafft, B. F. Becker, and M. Rehm Technical and physiological background of plasma volume measurement with indocyanine green: a clarification of misunderstandings J Appl Physiol, March 1, 2007; 102(3): 1235 - 1242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bjornson Granqvist, K. Ebefors, M. A. Saleem, P. W. Mathieson, B. Haraldsson, and J. S. Nystrom Podocyte proteoglycan synthesis is involved in the development of nephrotic syndrome Am J Physiol Renal Physiol, October 1, 2006; 291(4): F722 - F730. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. van den Berg and H. Vink Glycocalyx perturbation: cause or consequence of damage to the vasculature? Am J Physiol Heart Circ Physiol, June 1, 2006; 290(6): H2174 - H2175. [Full Text] [PDF]
|
|
|
|
|
|
I. Rubio-Gayosso, S. H. Platts, and B. R. Duling Reactive oxygen species mediate modification of glycocalyx during ischemia-reperfusion injury Am J Physiol Heart Circ Physiol, June 1, 2006; 290(6): H2247 - H2256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Nieuwdorp, H. L. Mooij, J. Kroon, B. Atasever, J. A.E. Spaan, C. Ince, F. Holleman, M. Diamant, R. J. Heine, J. B.L. Hoekstra, et al. Endothelial glycocalyx damage coincides with microalbuminuria in type 1 diabetes. Diabetes, April 1, 2006; 55(4): 1127 - 1132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Chen, D. Jaron, K. A. Barbee, and D. G. Buerk The influence of radial RBC distribution, blood velocity profiles, and glycocalyx on coupled NO/O2 transport J Appl Physiol, February 1, 2006; 100(2): 482 - 492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Mehta and A. B. Malik Signaling Mechanisms Regulating Endothelial Permeability Physiol Rev, January 1, 2006; 86(1): 279 - 367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Jeansson and B. Haraldsson Morphological and functional evidence for an important role of the endothelial cell glycocalyx in the glomerular barrier Am J Physiol Renal Physiol, January 1, 2006; 290(1): F111 - F116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Gouverneur, J. A. E. Spaan, H. Pannekoek, R. D. Fontijn, and H. Vink Fluid shear stress stimulates incorporation of hyaluronan into endothelial cell glycocalyx Am J Physiol Heart Circ Physiol, January 1, 2006; 290(1): H458 - H452. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. A. van Haaren, E. VanBavel, H. Vink, and J. A. E. Spaan Charge modification of the endothelial surface layer modulates the permeability barrier of isolated rat mesenteric small arteries Am J Physiol Heart Circ Physiol, December 1, 2005; 289(6): H2503 - H2507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. G. E. VanTeeffelen, S. Dekker, D. S. Fokkema, M. Siebes, H. Vink, and J. A. E. Spaan Hyaluronidase treatment of coronary glycocalyx increases reactive hyperemia but not adenosine hyperemia in dog hearts Am J Physiol Heart Circ Physiol, December 1, 2005; 289(6): H2508 - H2513. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Pries and T. W. Secomb Microvascular blood viscosity in vivo and the endothelial surface layer Am J Physiol Heart Circ Physiol, December 1, 2005; 289(6): H2657 - H2664. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Bruegger, M. Jacob, M. Rehm, M. Loetsch, U. Welsch, P. Conzen, and B. F. Becker Atrial natriuretic peptide induces shedding of endothelial glycocalyx in coronary vascular bed of guinea pig hearts Am J Physiol Heart Circ Physiol, November 1, 2005; 289(5): H1993 - H1999. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Zuurbier, C. Demirci, A. Koeman, H. Vink, and C. Ince Short-term hyperglycemia increases endothelial glycocalyx permeability and acutely decreases lineal density of capillaries with flowing red blood cells J Appl Physiol, October 1, 2005; 99(4): 1471 - 1476. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Ono, R. Mizuno, and T. Ohhashi Effective permeability of hydrophilic substances through walls of lymph vessels: roles of endothelial barrier Am J Physiol Heart Circ Physiol, October 1, 2005; 289(4): H1676 - H1682. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. A. Wills, N. M. Dung, H. T. Loan, D. T.H. Tam, T. T.N. Thuy, L. T.T. Minh, T. V. Diet, N. T. Hao, N. V. Chau, K. Stepniewska, et al. Comparison of Three Fluid Solutions for Resuscitation in Dengue Shock Syndrome N. Engl. J. Med., September 1, 2005; 353(9): 877 - 889. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. F. Flessner The transport barrier in intraperitoneal therapy Am J Physiol Renal Physiol, March 1, 2005; 288(3): F433 - F442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ueda, M. Shimomura, M. Ikeda, R. Yamaguchi, and K. Tanishita Effect of glycocalyx on shear-dependent albumin uptake in endothelial cells Am J Physiol Heart Circ Physiol, November 1, 2004; 287(5): H2287 - H2294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. W. Mulivor and H. H. Lipowsky Inflammation- and ischemia-induced shedding of venular glycocalyx Am J Physiol Heart Circ Physiol, May 1, 2004; 286(5): H1672 - H1680. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. H. Platts and B. R. Duling Adenosine A3 Receptor Activation Modulates the Capillary Endothelial Glycocalyx Circ. Res., January 9, 2004; 94(1): 77 - 82. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. A. van Haaren, E. VanBavel, H. Vink, and J. A. E. Spaan Localization of the permeability barrier to solutes in isolated arteries by confocal microscopy Am J Physiol Heart Circ Physiol, December 1, 2003; 285(6): H2848 - H2856. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Florian, J. R. Kosky, K. Ainslie, Z. Pang, R. O. Dull, and J. M. Tarbell Heparan Sulfate Proteoglycan Is a Mechanosensor on Endothelial Cells Circ. Res., November 14, 2003; 93 (10): e136 - e142. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-D. Mi, H. Ishihara, T. Sakai, and A. Matsuki Possible Overestimation of Indocyanine Green-Derived Plasma Volume Early After Induction of Anesthesia with Propofol/Fentanyl Anesth. Analg., November 1, 2003; 97(5): 1421 - 1427. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mochizuki, H. Vink, O. Hiramatsu, T. Kajita, F. Shigeto, J. A. E. Spaan, and F. Kajiya Role of hyaluronic acid glycosaminoglycans in shear-induced endothelium-derived nitric oxide release Am J Physiol Heart Circ Physiol, July 11, 2003; 285(2): H722 - H726. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Platts, J. Linden, and B. R. Duling Rapid modification of the glycocalyx caused by ischemia-reperfusion is inhibited by adenosine A2A receptor activation Am J Physiol Heart Circ Physiol, June 1, 2003; 284(6): H2360 - H2367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Gonzalez-Castillo, R. Rubio, and T. Zenteno-Savin Coronary flow-induced inotropism is modulated by binding of dextrans to the endothelial luminal surface Am J Physiol Heart Circ Physiol, April 1, 2003; 284(4): H1348 - H1357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Sorensson, A. Bjornson, M. Ohlson, B. J. Ballermann, and B. Haraldsson Synthesis of sulfated proteoglycans by bovine glomerular endothelial cells in culture Am J Physiol Renal Physiol, February 1, 2003; 284(2): F373 - F380. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. W. Mulivor and H. H. Lipowsky Role of glycocalyx in leukocyte-endothelial cell adhesion Am J Physiol Heart Circ Physiol, October 1, 2002; 283(4): H1282 - H1291. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Osterloh, U. Ewert, and A. R. Pries Interaction of albumin with the endothelial cell surface Am J Physiol Heart Circ Physiol, July 1, 2002; 283(1): H398 - H405. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. J. M. F. Willemsen, D. J. Duncker, R. Krams, M. A. Dijkman, R. R. Lamberts, P. Sipkema, and N. Westerhof Decrease in coronary vascular volume in systole augments cardiac contraction Am J Physiol Heart Circ Physiol, August 1, 2001; 281(2): H731 - H737. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Constantinescu, H. Vink, and J. A. E. Spaan Elevated capillary tube hematocrit reflects degradation of endothelial cell glycocalyx by oxidized LDL Am J Physiol Heart Circ Physiol, March 1, 2001; 280(3): H1051 - H1057. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. B. S. Henry and B. R. Duling TNF-alpha increases entry of macromolecules into luminal endothelial cell glycocalyx Am J Physiol Heart Circ Physiol, December 1, 2000; 279(6): H2815 - H2823. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
