AJP - Heart AJP: Endocrinology and Metabolism
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 278: H444-H451, 2000;
0363-6135/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (5)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wagner, M. B.
Right arrow Articles by Joyner, R. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wagner, M. B.
Right arrow Articles by Joyner, R. W.
Vol. 278, Issue 2, H444-H451, February 2000

Measurements of calcium transients in ventricular cells during discontinuous action potential conduction

Mary B. Wagner, Yang-Gan Wang, Rajiv Kumar, David A. Golod, William N. Goolsby, and Ronald W. Joyner

Todd Franklin Cardiac Research Laboratory, The Children's Heart Center, Department of Pediatrics, Emory University, Atlanta, Georgia 30322

The L-type calcium current (ICa) is important in sustaining propagation during discontinuous conduction. In addition, ICa is altered during discontinuous conduction, which may result in changes in the intracellular calcium transient. To study this, we have combined the ability to monitor intracellular calcium concentration ([Ca2+]i) in an isolated cardiac cell using confocal scanning laser fluorescence microscopy with our "coupling clamp" technique, which allows action potential propagation from the real cell to a real-time simulation of a model cell. Coupling a real cell to a model cell with a value of coupling conductance (GC = 8 nS) just above the critical value for action potential propagation results in both an increased amplitude and an increased rate of rise of the calcium transient. Similar but smaller changes in the calcium transient are caused by increasing GC to 20 nS. The increase of [Ca2+]i by discontinuous conduction is less than the increase of ICa, which may indicate that much of [Ca2+]i is the result of calcium released from the sarcoplasmic reticulum rather than the integration of ICa.

confocal laser scanning microscopy; fluo 3; coupling clamp


This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
H. E. D. J. ter Keurs and P. A. Boyden
Calcium and Arrhythmogenesis
Physiol Rev, April 1, 2007; 87(2): 457 - 506.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
R. Wilders
Dynamic clamp: a powerful tool in cardiac electrophysiology
J. Physiol., October 15, 2006; 576(2): 349 - 359.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
J.-M. Goaillard and E. Marder
Dynamic Clamp Analyses of Cardiac, Endocrine, and Neural Function
Physiology, June 1, 2006; 21(3): 197 - 207.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online