AJP - Heart AJP: Endocrinology and Metabolism
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 278: H670-H676, 2000;
0363-6135/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (31)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Boixel, C.
Right arrow Articles by Hatem, S. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Boixel, C.
Right arrow Articles by Hatem, S. N.
Vol. 278, Issue 2, H670-H676, February 2000

RAPID COMMUNICATION
Tyrosine kinase and protein kinase C regulate L-type Ca2+ current cooperatively in human atrial myocytes

Christophe Boixel1, Sophie Tessier1, Yves Pansard2, Loïc Lang-Lazdunski2, Jean-Jacques Mercadier1, and Stephane N. Hatem1

1 Institut National de la Santé et de la Recherche Médicale, Unité 460, Faculté de Médecine Xavier Bichat, Paris; and 2 Service de Chirurgie Cardiaque, Hôpital Xavier Bichat, 75018 Paris, France

The effects of tyrosine protein kinases (TK) on the L-type Ca2+ current (ICa) were examined in whole cell patch-clamped human atrial myocytes. The TK inhibitors genistein (50 µM), lavendustin A (50 µM), and tyrphostin 23 (50 µM) stimulated ICa by 132 ± 18% (P < 0.001), 116 ± 18% (P < 0.05), and 60 ± 6% (P < 0.001), respectively. After ICa stimulation by genistein, external application of isoproterenol (1 µM) caused an additional increase in ICa. Dialyzing the cells with a protein kinase A inhibitor suppressed the effect of isoproterenol on ICa but not that of genistein. Inhibition of protein kinase C (PKC) by pretreatment of cells with 100 nM staurosporine or 100 nM calphostin C prevented the effects of genistein on ICa. The PKC activator phorbol 12-myristate 13-acetate (PMA), after an initial stimulation (75 ± 17%, P < 0.05), decreased ICa (-36 ± 5%, P < 0.001). Once the inhibitory effect of PMA on ICa had stabilized, genistein strongly stimulated the current (323 ± 25%, P < 0.05). Pretreating myocytes with genistein reduced the inhibitory effect of PMA on ICa. We conclude that, in human atrial myocytes, TK inhibit ICa via a mechanism that involves PKC.

human cardiac cells; whole cell patch clamp; L-type calcium channels; tyrosine kinase inhibitors


This article has been cited by other articles:


Home page
 Exp PhysiolHome page
S.-S. Zhou, L.-B. Zhang, W.-P. Sun, F.-C. Xiao, Y.-M. Zhou, Y.-J. Li, and D.-L. Li
Heart/Cardiac Muscle: Effects of monocarboxylic acid-derived Cl- channel blockers on depolarization-activated potassium currents in rat ventricular myocytes
Exp Physiol, May 1, 2007; 92(3): 549 - 559.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
M. A.G. van der Heyden, T. J.M. Wijnhoven, and T. Opthof
Molecular aspects of adrenergic modulation of cardiac L-type Ca2+ channels
Cardiovasc Res, January 1, 2005; 65(1): 28 - 39.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. M. Tipparaju, R. Kumar, Y. Wang, R. W. Joyner, and M. B. Wagner
Developmental differences in L-type calcium current of human atrial myocytes
Am J Physiol Heart Circ Physiol, May 1, 2004; 286(5): H1963 - H1969.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. W. Carter and N. L. Kanagy
Mechanism of enhanced calcium sensitivity and alpha 2-AR vasoreactivity in chronic NOS inhibition hypertension
Am J Physiol Heart Circ Physiol, January 1, 2003; 284(1): H309 - H316.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
K. D. Keef, J. R. Hume, and J. Zhong
Regulation of cardiac and smooth muscle Ca2+ channels (CaV1.2a,b) by protein kinases
Am J Physiol Cell Physiol, December 1, 2001; 281(6): C1743 - C1756.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. J. Davis, X. Wu, T. R. Nurkiewicz, J. Kawasaki, P. Gui, M. A. Hill, and E. Wilson
Regulation of ion channels by protein tyrosine phosphorylation
Am J Physiol Heart Circ Physiol, November 1, 2001; 281(5): H1835 - H1862.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C. Boixel, S. Dinanian, L. Lang-Lazdunski, J.-J. Mercadier, and S. N. Hatem
Characterization of effects of endothelin-1 on the L-type Ca2+ current in human atrial myocytes
Am J Physiol Heart Circ Physiol, August 1, 2001; 281(2): H764 - H773.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
T. J. Kamp and J. W. Hell
Regulation of Cardiac L-Type Calcium Channels by Protein Kinase A and Protein Kinase C
Circ. Res., December 8, 2000; 87(12): 1095 - 1102.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
K. J. Alden, P. H. Goldspink, S. W. Ruch, P. M. Buttrick, and J. Garcia
Enhancement of L-type Ca2+ current from neonatal mouse ventricular myocytes by constitutively active PKC-beta II
Am J Physiol Cell Physiol, April 1, 2002; 282(4): C768 - C774.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
C. Boixel, W. Gonzalez, L. Louedec, and S. N. Hatem
Mechanisms of L-Type Ca2+ Current Downregulation in Rat Atrial Myocytes During Heart Failure
Circ. Res., September 28, 2001; 89(7): 607 - 613.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online