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Am J Physiol Heart Circ Physiol 278: H688-H697, 2000;
0363-6135/00 $5.00
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Vol. 278, Issue 3, H688-H697, March 2000

Nonlinear changes of transmembrane potential caused by defibrillation shocks in strands of cultured myocytes

Vladimir G. Fast1, Stephan Rohr4, and Raymond E. Ideker1,2,3

Departments of 1 Biomedical Engineering, 2 Medicine, and 3 Physiology, University of Alabama at Birmingham, Birmingham, Alabama 35294; and 4 Department of Physiology, University of Bern, 3012 Bern, Switzerland

Organization of cardiac tissue into cell strands and layers has been implicated in changes of transmembrane potential (Delta Vm) during defibrillation. To determine the shock-induced Delta Vm in such structures, cell strands of variable width [strand width (SW) = 0.15-2 mm] were grown in culture. Uniform-field shocks with variable strength [shock strength (SS) = 2-50 V/cm] were applied across strands during the action potential (AP) plateau, and Delta Vm were measured optically. Three different types of Delta Vm were observed. Small Delta Vm [<40%AP amplitude (APA)] were linearly dependent on SS and SW and were symmetrically distributed about a strand centerline with maximal positive and negative Delta Vm on opposite strand sides being equal. Intermediate Delta Vm (<200%APA) were strongly asymmetric with negative Delta Vm > positive Delta Vm because of a negative time-dependent shift of Vm at the depolarized side of the strands. For large Delta Vm (>200%APA), a second time-dependent shift of Vm to more positive levels was observed in the hyperpolarized portions of strands, causing reduction of the Delta Vm asymmetry. We conclude that during application of shocks to cell strands during the AP plateau, passive changes of Vm were followed by two voltage- and time-dependent shifts of Vm, possibly reflecting changes of ionic currents or membrane electroporation.

stimulation; optical mapping; voltage-sensitive dyes; cell cultures


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