| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Human Physiology, School of Medicine, University of California, Davis, California 95616
We reported previously that increasing cAMP levels in endothelial cells attenuated ATP-induced increases in hydraulic conductivity (Lp), and that the activation of cGMP-dependent pathways was a necessary step to increase Lp in response to inflammatory mediators. The aim of the present study was to evaluate the role of basal levels of cAMP in microvessel permeability under resting conditions and to evaluate the cross talk between cAMP- and cGMP-dependent signaling mechanisms in regulation of microvessel permeability under stimulated conditions, using individually perfused microvessels from frog and rat mesenteries. We found that reducing cAMP levels by inhibition of adenylate cyclase or inhibiting cAMP-dependent protein kinase through the use of H-89 increased basal Lp in both frog and rat mesenteric venular microvessels. We also found that 8-bromocAMP (8-BrcAMP, 0.2 and 2 mM) was sufficient to attenuate or abolish the increases in Lp due to exposure of frog mesenteric venular microvessels to 8-BrcGMP (2 mM) and ATP (10 µM). Similarly, in rat mesenteric venular microvessels, application of 8-BrcAMP (2 mM) abolished the increases in Lp due to exposure to 8-BrcGMP alone (2 mM) or with the combination of bradykinin (1 nM). In addition, application of erythro-9-(2-hydroxy-3-nonyl)adenine, an inhibitor of cGMP-stimulated phosphodiesterase, significantly attenuated both 8-BrcGMP- and bradykinin-induced increases in Lp. These results demonstrate that basal levels of cAMP are critical to maintaining normal permeability under resting conditions, and that increased levels of cAMP are capable of overcoming the activation of cGMP-dependent pathways, therefore preventing increases in microvessel permeability. The balance between endothelial concentrations of these two opposing cyclic nucleotides controls microvessel permeability, and cAMP levels play a dominant role.
hydraulic conductivity; adenosine 3',5'-cyclic monophosphate analog; guanosine 3',5'-cyclic monophosphate analog; erythro-9-(2-hydroxy-3-nonyl)adenine-phosphodiesterase inhibitor; adenylate cyclase inhibitor
This article has been cited by other articles:
|
|
 |
|
  E. P. Schmidt, M. Damarla, O. Rentsendorj, L. E. Servinsky, B. Zhu, A. Moldobaeva, A. Gonzalez, P. M. Hassoun, and D. B. Pearse Soluble guanylyl cyclase contributes to ventilator-induced lung injury in mice Am J Physiol Lung Cell Mol Physiol, December 1, 2008; 295(6): L1056 - L1065. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Jiang, K. Wen, X. Zhou, D. Schwegler-Berry, V. Castranova, and P. He Three-dimensional localization and quantification of PAF-induced gap formation in intact venular microvessels Am J Physiol Heart Circ Physiol, August 1, 2008; 295(2): H898 - H906. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Puana, R. K. McAllister, F. A. Hunter, J. Warden, and E. W. Childs Morphine Attenuates Microvascular Hyperpermeability via a Protein Kinase A-Dependent Pathway Anesth. Analg., February 1, 2008; 106(2): 480 - 485. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. Morris and T. C. Skalak Acute exposure to a moderate strength static magnetic field reduces edema formation in rats Am J Physiol Heart Circ Physiol, January 1, 2008; 294(1): H50 - H57. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Schlegel, S. Burger, N. Golenhofen, U. Walter, D. Drenckhahn, and J. Waschke The role of VASP in regulation of cAMP- and Rac 1-mediated endothelial barrier stabilization Am J Physiol Cell Physiol, January 1, 2008; 294(1): C178 - C188. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Lorenowicz, M. Fernandez-Borja, and P. L. Hordijk cAMP Signaling in Leukocyte Transendothelial Migration Arterioscler Thromb Vasc Biol, May 1, 2007; 27(5): 1014 - 1022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wang and V. H. Huxley Adenosine A2A receptor modulation of juvenile female rat skeletal muscle microvessel permeability Am J Physiol Heart Circ Physiol, December 1, 2006; 291(6): H3094 - H3105. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. He, H. Zhang, L. Zhu, Y. Jiang, and X. Zhou Leukocyte-platelet aggregate adhesion and vascular permeability in intact microvessels: role of activated endothelial cells Am J Physiol Heart Circ Physiol, August 1, 2006; 291(2): H591 - H599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Charleston, R. Puana, R. K. McAllister, F. A. Hunter, and E. W. Childs Morphine sulfate attenuates hemorrhagic shock- induced hyperpermeability. Anesth. Analg., July 1, 2006; 103(1): 156 - 161. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Mehta and A. B. Malik Signaling Mechanisms Regulating Endothelial Permeability Physiol Rev, January 1, 2006; 86(1): 279 - 367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-h. Kim, N. R. Harris, and J. M. Tarbell Regulation of capillary hydraulic conductivity in response to an acute change in shear Am J Physiol Heart Circ Physiol, November 1, 2005; 289(5): H2126 - H2135. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zhu and P. He Platelet-activating factor increases endothelial [Ca2+]i and NO production in individually perfused intact microvessels Am J Physiol Heart Circ Physiol, June 1, 2005; 288(6): H2869 - H2877. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Glass, T. M. Pocock, F. E. Curry, and D. O. Bates Cytosolic Ca2+ concentration and rate of increase of the cytosolic Ca2+ concentration in the regulation of vascular permeability in Rana in vivo J. Physiol., May 1, 2005; 564(3): 817 - 827. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. H. Huxley, J. Wang, and S. P. Whitt Sexual dimorphism in the permeability response of coronary microvessels to adenosine Am J Physiol Heart Circ Physiol, April 1, 2005; 288(4): H2006 - H2013. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Waschke, F. E. Curry, R. H. Adamson, and D. Drenckhahn Regulation of actin dynamics is critical for endothelial barrier functions Am J Physiol Heart Circ Physiol, March 1, 2005; 288(3): H1296 - H1305. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Waschke, D. Drenckhahn, R. H. Adamson, H. Barth, and F. E. Curry cAMP protects endothelial barrier functions by preventing Rac-1 inhibition Am J Physiol Heart Circ Physiol, December 1, 2004; 287(6): H2427 - H2433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Bindewald, D. Gunduz, F. Hartel, S. C. Peters, C. Rodewald, S. Nau, M. Schafer, J. Neumann, H. M. Piper, and T. Noll Opposite effect of cAMP signaling in endothelial barriers of different origin Am J Physiol Cell Physiol, November 1, 2004; 287(5): C1246 - C1255. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. C. Satchell, K. L. Anderson, and P. W. Mathieson Angiopoietin 1 and Vascular Endothelial Growth Factor Modulate Human Glomerular Endothelial Cell Barrier Properties J. Am. Soc. Nephrol., March 1, 2004; 15(3): 566 - 574. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. H. Adamson, M. Zeng, G. N. Adamson, J. F. Lenz, and F. E. Curry PAF- and bradykinin-induced hyperpermeability of rat venules is independent of actin-myosin contraction Am J Physiol Heart Circ Physiol, June 5, 2003; 285(1): H406 - H417. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. B. Tarpey, D. R. Sawmiller, C. Kelly, W. J. Thompson, and M. I. Townsley Phosphodiesterase 3 activity is reduced in dog lung following pacing-induced heart failure Am J Physiol Lung Cell Mol Physiol, May 1, 2003; 284(5): L766 - L773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Creighton, N. Masada, D. M. F. Cooper, and T. Stevens Coordinate regulation of membrane cAMP by Ca2+-inhibited adenylyl cyclase and phosphodiesterase activities Am J Physiol Lung Cell Mol Physiol, January 1, 2003; 284(1): L100 - L107. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zeng, H. Zhang, C. Lowell, and P. He Tumor necrosis factor-alpha -induced leukocyte adhesion and microvessel permeability Am J Physiol Heart Circ Physiol, December 1, 2002; 283(6): H2420 - H2430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. He, J. Wang, and M. Zeng Leukocyte adhesion and microvessel permeability Am J Physiol Heart Circ Physiol, May 1, 2000; 278(5): H1686 - H1694. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
