AJP - Heart Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 278: H1815-H1822, 2000;
0363-6135/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (37)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Curtis, S. B.
Right arrow Articles by Buchan, A. M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Curtis, S. B.
Right arrow Articles by Buchan, A. M. J.
Vol. 278, Issue 6, H1815-H1822, June 2000

Somatostatin receptor subtype expression and function in human vascular tissue

Susan B. Curtis1, Jeff Hewitt2, Svetlana Yakubovitz3, Alexander Anzarut1, York N. Hsiang1, and Alison M. J. Buchan3

Departments of 1 Surgery, 2 Biochemistry and Molecular Biology, and 3 Physiology, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3

In animal models the somatostatin analog angiopeptin inhibits intimal hyperplasia by acting primarily through somatostatin receptor 2 (SSTR-2). However, the results of clinical trials using angiopeptin have been disappointing. In this study we showed that human blood vessels express high levels of SSTR-1 with significantly lower levels of SSTR-2 and -4. Samples of normal veins and arteries, as well as atherosclerotic arteries, expressed predominantly SSTR-1. In addition, the levels of SSTR-1 varied between individuals, indicating that the vascular disease process may have affected SSTR gene expression. Immunocytochemical studies demonstrated that SSTR-1 was present in endothelial but not vascular smooth muscle cells. No evidence of SSTR-3 or -5 expression was detected in normal or diseased blood vessels. Two endothelial cell preparations, ECV304 and human umbilical vein endothelial cells, were investigated and shown to express only SSTR-1 and -4. Exposure of these cells to 10 nM somatostatin or 10 nM SSTR-1-specific agonist resulted in alterations to the actin cytoskeleton, as characterized by a loss of actin stress fibers coupled with an increase in lamellipodia formation at the plasma membrane. These results suggest that the lack of effectiveness of angiopeptin in humans may be due to the differential expression of SSTR-1 by human endothelial cells.

endothelial cells; lamellipodia; actin stress fibers; human umbilical vein endothelial cells


This article has been cited by other articles:


Home page
 Toxicol PatholHome page
M. Y. Wells, H. Voute, M.-O. Lonchampt, C. Fisch, V. Boulifard, and P. Picaut
Intimal Hyperplasia in Rats after Subcutaneous Injection of a Somatostatin Analog
Toxicol Pathol, February 1, 2009; 37(2): 235 - 243.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
M. Engstrom, J. Tomperi, K. El-Darwish, M. Ahman, J.-M. Savola, and S. Wurster
Superagonism at the Human Somatostatin Receptor Subtype 4
J. Pharmacol. Exp. Ther., January 1, 2005; 312(1): 332 - 338.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
B. Cauli, X.-K. Tong, A. Rancillac, N. Serluca, B. Lambolez, J. Rossier, and E. Hamel
Cortical GABA Interneurons in Neurovascular Coupling: Relays for Subcortical Vasoactive Pathways
J. Neurosci., October 13, 2004; 24(41): 8940 - 8949.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
H Reynaert, K Rombouts, A Vandermonde, D Urbain, U Kumar, P Bioulac-Sage, M Pinzani, J Rosenbaum, and A Geerts
Expression of somatostatin receptors in normal and cirrhotic human liver and in hepatocellular carcinoma
Gut, August 1, 2004; 53(8): 1180 - 1189.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online