AJP - Heart AJP: Cell Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 278: H1832-H1839, 2000;
0363-6135/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (32)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Welsh, D. G.
Right arrow Articles by Segal, S. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Welsh, D. G.
Right arrow Articles by Segal, S. S.
Vol. 278, Issue 6, H1832-H1839, June 2000

Role of EDHF in conduction of vasodilation along hamster cheek pouch arterioles in vivo

Donald G. Welsh and Steven S. Segal

The John B. Pierce Laboratory and Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06519

We tested whether local and conducted responses to ACh depend on factors released from endothelial cells (EC) in cheek pouch arterioles of anesthetized hamsters. ACh was delivered from a micropipette (1 s, 500 nA), while arteriolar diameter (rest, ~40 µm) was monitored at the site of application (local) and at 520 and 1,040 µm upstream (conducted). Under control conditions, ACh elicited local (22-65 µm) and conducted (14-44 µm) vasodilation. Indomethacin (10 µM) had no effect, whereas Nomega -nitro-L-arginine (100 µM) reduced local and conducted vasodilation by 5-8% (P < 0.05). Miconazole (10 µM) or 17-octadecynoic acid (17-ODYA; 10 µM) diminished local vasodilation by 15-20% and conducted responses by 50-70% (P < 0.05), suggesting a role for cytochrome P-450 (CYP) metabolites in arteriolar responses to ACh. Membrane potential (Em) was recorded in smooth muscle cells (SMC) and in EC identified with dye labeling. At rest (control Em, typically -30 mV), ACh evoked local (15-32 mV) and conducted (6-31 mV) hyperpolarizations in SMC and EC. Miconazole inhibited SMC and EC hyperpolarization, whereas 17-ODYA inhibited hyperpolarization of SMC but not of EC. Findings indicate that ACh-induced release of CYP metabolites from arteriolar EC evoke SMC hyperpolarization that contributes substantively to conducted vasodilation.

acetylcholine; endothelium-derived hyperpolarizing factor; cell-to-cell communication; cytochrome P-450 pathway; membrane potential; microcirculation; nitric oxide


This article has been cited by other articles:


Home page
 Cardiovasc ResHome page
C. de Wit
Different pathways with distinct properties conduct dilations in the microcirculation in vivo
Cardiovasc Res, October 31, 2009; (2009) cvp340v2.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
X. F. Figueroa and B. R. Duling
Dissection of two Cx37-independent conducted vasodilator mechanisms by deletion of Cx40: electrotonic versus regenerative conduction
Am J Physiol Heart Circ Physiol, November 1, 2008; 295(5): H2001 - H2007.
[Abstract] [Full Text] [PDF]

Home page
 StrokeHome page
A. C. Ngai, T.-S. Nguyen, J. R. Meno, and G. W. Britz
Postischemic Augmentation of Conducted Dilation in Cerebral Arterioles
Stroke, January 1, 2007; 38(1): 124 - 130.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. B. Samora, J. C. Frisbee, and M. A. Boegehold
Growth-dependent changes in endothelial factors regulating arteriolar tone
Am J Physiol Heart Circ Physiol, January 1, 2007; 292(1): H207 - H214.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. F. Brekke, W. F. Jackson, and S. S. Segal
Arteriolar smooth muscle Ca2+ dynamics during blood flow control in hamster cheek pouch
J Appl Physiol, July 1, 2006; 101(1): 307 - 315.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
A. Huang, D. Sun, A. Jacobson, M. A. Carroll, J. R. Falck, and G. Kaley
Epoxyeicosatrienoic Acids Are Released to Mediate Shear Stress-Dependent Hyperpolarization of Arteriolar Smooth Muscle
Circ. Res., February 18, 2005; 96(3): 376 - 383.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. P. Marrelli, M. S. Eckmann, and M. S. Hunte
Role of endothelial intermediate conductance KCa channels in cerebral EDHF-mediated dilations
Am J Physiol Heart Circ Physiol, October 1, 2003; 285(4): H1590 - H1599.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
I. Fleming
Bobbing Along on the Crest of a Wave: NO Ascends Hamster Cheek Pouch Arterioles
Circ. Res., July 11, 2003; 93(1): 9 - 11.
[Full Text] [PDF]

Home page
 Circ. Res.Home page
S. Budel, I. S. Bartlett, and S. S. Segal
Homocellular Conduction Along Endothelium and Smooth Muscle of Arterioles in Hamster Cheek Pouch: Unmasking an NO Wave
Circ. Res., July 11, 2003; 93(1): 61 - 68.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
K. A. Dora, J. Xia, and B. R. Duling
Endothelial cell signaling during conducted vasomotor responses
Am J Physiol Heart Circ Physiol, June 5, 2003; 285(1): H119 - H126.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
Y. Yashiro and B. R. Duling
Participation of intracellular Ca2+ stores in arteriolar conducted responses
Am J Physiol Heart Circ Physiol, June 5, 2003; 285(1): H65 - H73.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
B. Hoepfl, B. Rodenwaldt, U. Pohl, and C. de Wit
EDHF, but not NO or prostaglandins, is critical to evoke a conducted dilation upon ACh in hamster arterioles
Am J Physiol Heart Circ Physiol, September 1, 2002; 283(3): H996 - H1004.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
G. G. Emerson, T. O. Neild, and S. S. Segal
Conduction of hyperpolarization along hamster feed arteries: augmentation by acetylcholine
Am J Physiol Heart Circ Physiol, July 1, 2002; 283(1): H102 - H109.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
G. G. Emerson and S. S. Segal
Electrical Coupling Between Endothelial Cells and Smooth Muscle Cells in Hamster Feed Arteries : Role in Vasomotor Control
Circ. Res., September 15, 2000; 87(6): 474 - 479.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online