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Am J Physiol Heart Circ Physiol 279: H250-H259, 2000;
0363-6135/00 $5.00
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Vol. 279, Issue 1, H250-H259, July 2000

Electrotonic suppression of early afterdepolarizations in isolated rabbit Purkinje myocytes

Delilah J. Huelsing1, Kenneth W. Spitzer2, and Andrew E. Pollard1

1 Cardiac Rhythm Management Laboratory and Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, Alabama 35294; and 2 Nora Eccles Harrison Cardiovascular Research and Training Institute, University of Utah School of Medicine, Salt Lake City, Utah 84112

Many studies suggest that early afterdepolarizations (EADs) arising from Purkinje fibers initiate triggered arrhythmias under pathological conditions. However, electrotonic interactions between Purkinje and ventricular myocytes may either facilitate or suppress EAD formation at the Purkinje-ventricular interface. To determine conditions that facilitated or suppressed EADs during Purkinje-ventricular interactions, we coupled single Purkinje myocytes and aggregates isolated from rabbit hearts to a passive model cell via an electronic circuit with junctional resistance (Rj). The model cell had input resistance (Rm,v) of 50 MOmega , capacitance of 39 pF, and a variable rest potential (Vrest,v). EADs were induced in Purkinje myocytes during superfusion with 1 µM isoproterenol. Coupling at high Rj to normally polarized Vrest,v established a repolarizing coupling current during all phases of the Purkinje action potential. This coupling current preferentially suppressed EADs in single cells with mean membrane resistance (Rm,p) of 297 MOmega , whereas EAD suppression in larger aggregates with mean Rm,p of 80 MOmega required larger coupling currents. In contrast, coupling to elevated Vrest,v established a depolarizing coupling current during late phase 2, phase 3, and phase 4 that facilitated EAD formation and induced spontaneous activity in single Purkinje myocytes and aggregates. These results have important implications for arrhythmogenesis in the infarcted heart when reduction of the ventricular mass due to scarring alters the Rm,p-to-Rm,v ratio and in the ischemic heart when injury currents are established during coupling between polarized Purkinje myocytes and depolarized ventricular myocytes.

Purkinje-ventricular junction; injury current; membrane resistance


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