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Am J Physiol Heart Circ Physiol 279: H852-H856, 2000;
0363-6135/00 $5.00
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Vol. 279, Issue 2, H852-H856, August 2000

SPECIAL COMMUNICATION
NO metabolites accumulate in erythrocytes in proportion to carbon dioxide and bicarbonate concentration

Fabio A. Recchia, Traci R. Vogel, and Thomas H. Hintze

Department of Physiology, New York Medical College, Valhalla, New York 10595

It is not known whether the ratio between the concentrations of NO metabolites (NOx) in plasma (pNOx) and in erythrocytes (eNOx) is constant or correlates with chemical parameters of the blood. We measured pH, PO2, and PCO2 and calculated bicarbonate concentration in 19 blood samples from the aorta, coronary sinus, and leg veins of 7 dogs. Erythrocytes were then separated by centrifugation and lysed with distilled water, and the lysate was ultrafiltered with a molecular cutoff of 50 kDa to remove the hemoglobin. NOx were measured in plasma and in the ultrafiltrate. NOx concentration was higher in erythrocytes, with eNOx/pNOx ranging from 4.38 to 14.60. Linear and significant correlations were found between the natural logarithm of eNOx/pNOx and PCO2 (r = 0.70, P < 0.001) or bicarbonate concentration (r = 0.72, P < 0.001). These results demonstrate, for the first time, that plasma NOx cannot be considered as a constant fraction of the total NOx in blood but varies dramatically in proportion to the CO2/bicarbonate concentration. To prevent an underestimation of venous-arterial difference of NOx across organs, NOx should be measured in whole blood.

nitrate; nitric oxide metabolite measurements


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