Vol. 279, Issue 3, H1022-H1028, September 2000
Comparison of blood-brain barrier permeability in mice and
rats using in situ brain perfusion technique
Hideyasu
Murakami,
Hitomi
Takanaga,
Hirotami
Matsuo,
Hisakazu
Ohtani, and
Yasufumi
Sawada
Department of Biopharmaceutics, Graduate School of Pharmaceutical
Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka
812-8582, Japan
Here we present a method for
measuring the permeability coefficient-surface area product
(PS) values at the blood-brain barrier in mice, using the in
situ brain perfusion technique originally developed for rats by
Takasato et al. (Am J Physiol Heart Circ Physiol 247:
H484-H493, 1984). Retrograde infusion into the right external
carotid artery increased the carotid perfusion pressure in proportion
to the perfusion rate. Intravascular volume and cerebral perfusion
fluid flow at a perfusion rate of 1.0 ml/min in mice were similar to
those in rats. In addition, the contribution of systemic blood to total
flow in the hemisphere was small (only 3.2%). These findings indicated
that this perfusion rate is suitable for mice. The PS values
of more than 20 different compounds were determined in mice by using
the in situ brain perfusion technique, and comparisons were made with
data from rats. There was a close relationship (1:1) between the
PS values in mice and rats, indicating that brain capillary
permeabilities are similar in mice and rats.
carotid perfusion pressure; vascular volume; cerebral perfusion
fluid flow; contribution of systemic blood to total flow; permeability
coefficient-surface area product
