AJP - Heart Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 279: H939-H945, 2000;
0363-6135/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (35)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mustapha, S.
Right arrow Articles by Kirshenbaum, L. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mustapha, S.
Right arrow Articles by Kirshenbaum, L. A.
Vol. 279, Issue 3, H939-H945, September 2000

A direct requirement of nuclear factor-kappa B for suppression of apoptosis in ventricular myocytes

Shareef Mustapha, Alla Kirshner, Danielle De Moissac, and Lorrie A. Kirshenbaum

Faculty of Medicine, Department of Physiology, Institute of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, University of Manitoba, Winnipeg, Manitoba, Canada R2H 2A6

Nuclear factor-kappa B (NF-kappa B) is a ubiquitously expressed cellular factor regulated by the cytoplasmic factor inhibitor protein kappa Balpha (Ikappa Balpha ). Activation of NF-kappa B by cytokines, including tumor necrosis factor-alpha (TNF-alpha ), requires the phosphorylation and degradation of Ikappa Balpha . An anti-apoptotic role for NF-kappa B has recently been suggested. In the present study, we ascertained whether death-promoting signals and apoptosis mediated by TNF-alpha are suppressed by NF-kappa B in postnatal ventricular myocytes. Stimulation of myocytes with TNF-alpha resulted in a 12.1-fold increase (P < 0.01) in NF-kappa B-dependent gene transcription and DNA binding compared with controls. This was accompanied by a corresponding increase in the NF-kappa B target protein A20 as determined by Western blot analysis. Vital staining revealed that TNF-alpha was not cytotoxic to myocytes and did not provoke apoptosis. Adenovirus-mediated delivery of a nonphosphorylatable form of Ikappa Balpha to inactivate NF-kappa B prevented TNF-alpha -stimulated NF-kappa B-dependent gene transcription and nuclear NF-kappa B DNA binding. Importantly, myocytes stimulated with TNF-alpha and defective for NF-kappa B activation resulted in a 2.2-fold increase (P < 0.001) in apoptosis. To our knowledge, the data provide the first indication that a functional NF-kappa B signaling pathway is crucial for suppressing death-promoting signals mediated by TNF-alpha in ventricular myocytes.

adenovirus; inflammation; cytokines; heart failure


This article has been cited by other articles:


Home page
 Circ. Res.Home page
N. Yurkova, J. Shaw, K. Blackie, D. Weidman, R. Jayas, B. Flynn, and L. A. Kirshenbaum
The Cell Cycle Factor E2F-1 Activates Bnip3 and the Intrinsic Death Pathway in Ventricular Myocytes
Circ. Res., February 29, 2008; 102(4): 472 - 479.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
H.-L. Li, M.-L. Zhuo, D. Wang, A.-B. Wang, H. Cai, L.-H. Sun, Q. Yang, Y. Huang, Y.-S. Wei, P. P. Liu, et al.
Targeted Cardiac Overexpression of A20 Improves Left Ventricular Performance and Reduces Compensatory Hypertrophy After Myocardial Infarction
Circulation, April 10, 2007; 115(14): 1885 - 1894.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
W. Chao, Y. Shen, L. Li, H. Zhao, S. E. Meiler, S. A. Cook, and A. Rosenzweig
Fas-associated death-domain protein inhibits TNF-{alpha} mediated NF-{kappa}B activation in cardiomyocytes
Am J Physiol Heart Circ Physiol, November 1, 2005; 289(5): H2073 - H2080.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
U. Haussler, G. von Wichert, R. M. Schmid, F. Keller, and G. Schneider
Epidermal growth factor activates nuclear factor-{kappa}B in human proximal tubule cells
Am J Physiol Renal Physiol, October 1, 2005; 289(4): F808 - F815.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. Brown, M. McGuinness, T. Wright, X. Ren, Y. Wang, G. P. Boivin, H. Hahn, A. M. Feldman, and W. K. Jones
Cardiac-specific blockade of NF-{kappa}B in cardiac pathophysiology: differences between acute and chronic stimuli in vivo
Am J Physiol Heart Circ Physiol, July 1, 2005; 289(1): H466 - H476.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
K. K. Meldrum, A. L. Burnett, X. Meng, R. Misseri, M. B.K. Shaw, J. P. Gearhart, and D. R. Meldrum
Liposomal Delivery of Heat Shock Protein 72 Into Renal Tubular Cells Blocks Nuclear Factor-{kappa}B Activation, Tumor Necrosis Factor-{alpha} Production, and Subsequent Ischemia-Induced Apoptosis
Circ. Res., February 21, 2003; 92(3): 293 - 299.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C. Steenbergen, C. A. Afshari, J. G. Petranka, J. Collins, K. Martin, L. Bennett, A. Haugen, P. Bushel, and E. Murphy
Alterations in apoptotic signaling in human idiopathic cardiomyopathic hearts in failure
Am J Physiol Heart Circ Physiol, January 1, 2003; 284(1): H268 - H276.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
K. M. Regula, K. Ens, and L. A. Kirshenbaum
Inducible Expression of BNIP3 Provokes Mitochondrial Defects and Hypoxia-Mediated Cell Death of Ventricular Myocytes
Circ. Res., August 9, 2002; 91(3): 226 - 231.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol PatholHome page
H. Roger Brown, Hong Ni, G. Benavides, L. Yoon, K. Hyder, J. Giridhar, G. Gardner, R. D. Tyler, and K. T. Morgan
Correlation of Simultaneous Differential Gene Expression in the Blood and Heart with Known Mechanisms of Adriamycin-Induced Cardiomyopathy in the Rat
Toxicol Pathol, June 1, 2002; 30(4): 452 - 469.
[Abstract] [PDF]

Home page
 FASEB J.Home page
B. ZINGARELLI, P. W. HAKE, Z. YANG, M. O'CONNOR, A. DENENBERG, and H. R. WONG
Absence of inducible nitric oxide synthase modulates early reperfusion-induced NF-{kappa}B and AP-1 activation and enhances myocardial damage
FASEB J, March 1, 2002; 16(3): 327 - 342.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
G. Wright, I. S. Singh, J. D. Hasday, I. K. Farrance, G. Hall, A. S. Cross, and T. B. Rogers
Endotoxin stress-response in cardiomyocytes: NF-kappa B activation and tumor necrosis factor-alpha expression
Am J Physiol Heart Circ Physiol, March 1, 2002; 282(3): H872 - H879.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. D. Southall, J. S. Isenberg, H. Nakshatri, Q. Yi, Y. Pei, D. F. Spandau, and J. B. Travers
The Platelet-activating Factor Receptor Protects Epidermal Cells from Tumor Necrosis Factor (TNF) alpha and TNF-related Apoptosis-inducing Ligand-induced Apoptosis through an NF-kappa B-dependent Process
J. Biol. Chem., November 30, 2001; 276(49): 45548 - 45554.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
K. K. Meldrum, D. R. Meldrum, K. L. Hile, E. B. Yerkes, A. Ayala, M. P. Cain, R. C. Rink, A. J. Casale, and M. A. Kaefer
p38 MAPK mediates renal tubular cell TNF-{alpha} production and TNF-{alpha}-dependent apoptosis during simulated ischemia
Am J Physiol Cell Physiol, August 1, 2001; 281(2): C563 - C570.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Frantz, R. A. Kelly, and T. Bourcier
Role of TLR-2 in the Activation of Nuclear Factor kappa B by Oxidative Stress in Cardiac Myocytes
J. Biol. Chem., February 9, 2001; 276(7): 5197 - 5203.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online