AJP - Heart Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 279: H1679-H1689, 2000;
0363-6135/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (63)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, R. C. X.
Right arrow Articles by Bolli, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, R. C. X.
Right arrow Articles by Bolli, R.
Vol. 279, Issue 4, H1679-H1689, October 2000

PKCepsilon modulates NF-kappa B and AP-1 via mitogen-activated protein kinases in adult rabbit cardiomyocytes

Richard C. X. Li1, Peipei Ping1,2, Jun Zhang2, William B. Wead2, Xinan Cao2, Jiuming Gao2, Yuting Zheng2, Shuang Huang3, Jiahuai Han3, and Roberto Bolli1,2

1 Experimental Research Laboratory, Division of Cardiology, 2 Department of Physiology and Biophysics, University of Louisville and the Jewish Hospital Heart and Lung Research Institute, Louisville, Kentucky 40202; and 3 Division of Immunology, Scripps Institute of Molecular Medicine, La Jolla, California 92037

We have previously shown that protein kinase C (PKC)-epsilon , nuclear factor (NF)-kappa B, and mitogen-activated protein kinases (MAPKs) are essential signaling elements in ischemic preconditioning. In the present study, we examined whether activation of PKCepsilon affects the activation of NF-kappa B in cardiac myocytes and whether MAPKs are mediators of this signaling event. Activation of PKCepsilon (+108% above control) in adult rabbit cardiomyocytes to a degree that has been previously shown to protect myocytes against hypoxic injury increased the DNA-binding activity of NF-kappa B (+164%) and activator protein (AP)-1 (+127%) but not that of Elk-1. Activation of PKCeta did not have an effect on these transcription factors. Activation of PKCepsilon also enhanced the phosphorylation activities of the p44/p42 MAPKs and the p54/p46 c-Jun NH2-terminal kinases (JNKs). PKCepsilon -induced activation of NF-kappa B and AP-1 was completely abolished by inhibition of the p44/p42 MAPK pathway with PD98059 and by inhibition of the p54/p46 JNK pathway with a dominant negative mutant of MAPK kinase-4, indicating that both signaling pathways are necessary. Taken together, these data identify NF-kappa B and AP-1 as downstream targets of PKCepsilon , thereby establishing a molecular link between activation of PKCepsilon and activation of NF-kappa B and AP-1 in cardiomyocytes. The results further demonstrate that both the p44/p42 MAPK and the p54/p46 JNK signaling pathways are essential mediators of this event.

protein kinase Cepsilon ; activator protein-1; nuclear factor-kappa B


This article has been cited by other articles:


Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
C. Rask-Madsen and G. L. King
Differential Regulation of VEGF Signaling by PKC-{alpha} and PKC-{epsilon} in Endothelial Cells
Arterioscler. Thromb. Vasc. Biol., May 1, 2008; 28(5): 919 - 924.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
N. J. Brown
Aldosterone and Vascular Inflammation
Hypertension, February 1, 2008; 51(2): 161 - 167.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
T. A. Wyatt, R. E. Slager, J. DeVasure, B. W. Auvermann, M. L. Mulhern, S. Von Essen, T. Mathisen, A. A. Floreani, and D. J. Romberger
Feedlot dust stimulation of interleukin-6 and -8 requires protein kinase C{varepsilon} in human bronchial epithelial cells
Am J Physiol Lung Cell Mol Physiol, November 1, 2007; 293(5): L1163 - L1170.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. Guo, T. Nguyen, M. Ogbi, H. Tawfik, G. Ma, Q. Yu, R. W. Caldwell, and J. A. Johnson
Protein kinase C-{varepsilon} coimmunoprecipitates with cytochrome oxidase subunit IV and is associated with improved cytochrome-c oxidase activity and cardioprotection
Am J Physiol Heart Circ Physiol, October 1, 2007; 293(4): H2219 - H2230.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
R. M. Bell, J. E. Clark, D. J. Hearse, and M. J. Shattock
Reperfusion kinase phosphorylation is essential but not sufficient in the mediation of pharmacological preconditioning: Characterisation in the bi-phasic profile of early and late protection
Cardiovasc Res, January 1, 2007; 73(1): 153 - 163.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
K. Inagaki, E. Churchill, and D. Mochly-Rosen
Epsilon protein kinase C as a potential therapeutic target for the ischemic heart
Cardiovasc Res, May 1, 2006; 70(2): 222 - 230.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
D. J. Hausenloy and D. M. Yellon
Survival kinases in ischemic preconditioning and postconditioning
Cardiovasc Res, May 1, 2006; 70(2): 240 - 253.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
R. J. Diaz and G. J. Wilson
Studying ischemic preconditioning in isolated cardiomyocyte models
Cardiovasc Res, May 1, 2006; 70(2): 286 - 296.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
E. McGregor and M. J. Dunn
Proteomics of the Heart: Unraveling Disease
Circ. Res., February 17, 2006; 98(3): 309 - 321.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
A. Denys, A. Hichami, and N. A. Khan
n-3 PUFAs modulate T-cell activation via protein kinase C-{alpha} and -{varepsilon} and the NF-{kappa}B signaling pathway
J. Lipid Res., April 1, 2005; 46(4): 752 - 758.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
T. Date, S. Mochizuki, A. J. Belanger, M. Yamakawa, Z. Luo, K. A. Vincent, S. H. Cheng, R. J. Gregory, and C. Jiang
Expression of constitutively stable hybrid hypoxia-inducible factor-1{alpha} protects cultured rat cardiomyocytes against simulated ischemia-reperfusion injury
Am J Physiol Cell Physiol, February 1, 2005; 288(2): C314 - C320.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
M.-C. Beauchamp, S.-E. Michaud, L. Li, M. R. Sartippour, and G. Renier
Advanced glycation end products potentiate the stimulatory effect of glucose on macrophage lipoprotein lipase expression
J. Lipid Res., September 1, 2004; 45(9): 1749 - 1757.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
R. Medeiros, D. A. Cabrini, J. Ferreira, E. S. Fernandes, M. A.S. Mori, J. B. Pesquero, M. Bader, M. C.W. Avellar, M. M. Campos, and J. B. Calixto
Bradykinin B1 Receptor Expression Induced by Tissue Damage in the Rat Portal Vein: A Critical Role for Mitogen-Activated Protein Kinase and Nuclear Factor-{kappa}B Signaling Pathways
Circ. Res., May 28, 2004; 94(10): 1375 - 1382.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
L. Li, T. Sawamura, and G. Renier
Glucose Enhances Human Macrophage LOX-1 Expression: Role for LOX-1 in Glucose-Induced Macrophage Foam Cell Formation
Circ. Res., April 16, 2004; 94(7): 892 - 901.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
A. Das, R. Ockaili, F. Salloum, and R. C. Kukreja
Protein kinase C plays an essential role in sildenafil-induced cardioprotection in rabbits
Am J Physiol Heart Circ Physiol, April 1, 2004; 286(4): H1455 - H1460.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. Zhang, P. Ping, T. M. Vondriska, X.-L. Tang, G.-W. Wang, E. M. Cardwell, and R. Bolli
Cardioprotection involves activation of NF-{kappa}B via PKC-dependent tyrosine and serine phosphorylation of I{kappa}B-{alpha}
Am J Physiol Heart Circ Physiol, October 1, 2003; 285(4): H1753 - H1758.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
L. Li, T. Sawamura, and G. Renier
Glucose Enhances Endothelial LOX-1 Expression: Role for LOX-1 in Glucose-Induced Human Monocyte Adhesion to Endothelium
Diabetes, July 1, 2003; 52(7): 1843 - 1850.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. R. Morris, R. D. Lutz, H.-S. Choi, T. Kamitani, K. Chmura, and E. D. Chan
Role of the NF-{kappa}B Signaling Pathway and {kappa}B cis-Regulatory Elements on the IRF-1 and iNOS Promoter Regions in Mycobacterial Lipoarabinomannan Induction of Nitric Oxide
Infect. Immun., March 1, 2003; 71(3): 1442 - 1452.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
A. Castrillo, D. J. Pennington, F. Otto, P. J. Parker, M. J. Owen, and L. Bosca
Protein Kinase C{epsilon} Is Required for Macrophage Activation and Defense Against Bacterial Infection
J. Exp. Med., October 29, 2001; 194(9): 1231 - 1242.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
T. M. Vondriska, J. B. Klein, and P. Ping
Use of functional proteomics to investigate PKC{epsilon}-mediated cardioprotection: the signaling module hypothesis
Am J Physiol Heart Circ Physiol, April 1, 2001; 280(4): H1434 - H1441.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. M. Pass, Y. Zheng, W. B. Wead, J. Zhang, R. C. X. Li, R. Bolli, and P. Ping
PKC{epsilon} activation induces dichotomous cardiac phenotypes and modulates PKC{epsilon}-RACK interactions and RACK expression
Am J Physiol Heart Circ Physiol, March 1, 2001; 280(3): H946 - H955.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
R. Bolli
The Late Phase of Preconditioning
Circ. Res., November 24, 2000; 87(11): 972 - 983.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
T. M. Vondriska, J. Zhang, C. Song, X.-L. Tang, X. Cao, C. P. Baines, J. M. Pass, S. Wang, R. Bolli, and P. Ping
Protein Kinase C {epsilon}-Src Modules Direct Signal Transduction in Nitric Oxide-Induced Cardioprotection : Complex Formation as a Means for Cardioprotective Signaling
Circ. Res., June 22, 2001; 88(12): 1306 - 1313.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online