AJP - Heart Calcium Transients and Cell-Sarcomere
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 279: H1830-H1838, 2000;
0363-6135/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (16)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chai, Y.-C.
Right arrow Articles by Chisolm, G. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chai, Y.-C.
Right arrow Articles by Chisolm, G. M.
Vol. 279, Issue 4, H1830-H1838, October 2000

Relationship of molecular structure to the mechanism of lysophospholipid-induced smooth muscle cell proliferation

Yuh-Cherng Chai, David G. Binion, and Guy M. Chisolm

Department of Cell Biology, Lerner Research Institute of the Cleveland Clinic Foundation, Cleveland, Ohio 44195

We previously reported that oxidized low-density lipoprotein and one of its constituents, lysophosphatidylcholine (lysoPC), caused smooth muscle cell proliferation that was inhibitable by vitamin E and by a neutralizing antibody against basic fibroblast growth factor-2 (FGF-2). We now show that the mitogenic activity of lysolipids is highly dependent on structure. Phospholipids with palmitoyl fatty acid and phosphocholine induced DNA synthesis optimally. Shorter and longer fatty acids were significantly less potent, as were phosphoserine and phosphoethanolamine head groups. Structurally related phospholipids [platelet-activating factor (PAF) and lysoPAF] were also mitogens and acted via an analogous FGF-2-dependent, vitamin E-inhibitable mechanism. The mechanism of lysoPC stimulation was distinct from that of another phospholipid mitogen, lysophosphatidic acid (lysoPA), in that lysoPC stimulation was not pertussis toxin inhibitable. Furthermore, lysoPA stimulation was not inhibitable by vitamin E. Despite its distinct cellular pathway for stimulation, lysoPA also ultimately led to FGF-2 release. Our data show that specific structural attributes of lysoPC, PAF, and lysoPAF enable these agents to mediate smooth muscle cell release of FGF-2, which in turn stimulates proliferation.

basic fibroblast growth factor; lysophosphatidylcholine; lysophosphatidic acid; platelet-activating factor; vitamin E


This article has been cited by other articles:


Home page
 Mol. Pharmacol.Home page
E. J. Welch, R. P. Naikawadi, Z. Li, P. Lin, S. Ishii, T. Shimizu, C. Tiruppathi, X. Du, P. V. Subbaiah, and R. D. Ye
Opposing Effects of Platelet-Activating Factor and Lyso-Platelet-Activating Factor on Neutrophil and Platelet Activation
Mol. Pharmacol., January 1, 2009; 75(1): 227 - 234.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
N. Gluck, O. Schwob, M. Krimsky, and S. Yedgar
Activation of cytosolic phospholipase A2 and fatty acid transacylase is essential but not sufficient for thrombin-induced smooth muscle cell proliferation
Am J Physiol Cell Physiol, June 1, 2008; 294(6): C1597 - C1603.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
A. Damirin, H. Tomura, M. Komachi, J.-P. Liu, C. Mogi, M. Tobo, J.-Q. Wang, T. Kimura, A. Kuwabara, Y. Yamazaki, et al.
Role of lipoprotein-associated lysophospholipids in migratory activity of coronary artery smooth muscle cells
Am J Physiol Heart Circ Physiol, May 1, 2007; 292(5): H2513 - H2522.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
N. Auge, O. Rebai, J. Lepetit-Thevenin, N. Bruneau, J.-C. Thiers, E. Mas, D. Lombardo, A. Negre-Salvayre, and A. Verine
Pancreatic Bile Salt-Dependent Lipase Induces Smooth Muscle Cells Proliferation
Circulation, July 8, 2003; 108(1): 86 - 91.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online