AJP - Heart pressure measurements
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


Am J Physiol Heart Circ Physiol 279: H2143-H2150, 2000;
0363-6135/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (18)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schäfer, C.
Right arrow Articles by Piper, H. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schäfer, C.
Right arrow Articles by Piper, H. M.
Vol. 279, Issue 5, H2143-H2150, November 2000

Inhibition of NHE protects reoxygenated cardiomyocytes independently of anoxic Ca2+ overload and acidosis

C. Schäfer, Y. V. Ladilov, M. Schäfer, and H. M. Piper

Physiologisches Institut, Justus Liebig Universität Giessen, D-35392 Giessen, Germany

We investigated the question of whether inhibition of the Na+/H+ exchanger (NHE) during ischemia is protective due to reduction of cytosolic Ca2+ accumulation or enhanced acidosis in cardiomyocytes. Additionally, the role of the Na+-HCO3- symporter (NBS) was investigated. Adult rat cardiomyocytes were exposed to simulated ischemia and reoxygenation. Cytosolic pH [2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF)], Ca2+ (fura 2), Na+ [sodium-binding benzolfuran isophthatlate (SBFI)], and cell length were measured. NHE was inhibited with 3 µmol/l HOE 642 or 1 µmol/l 5-(N-ethyl-N-isopropyl)-amiloride (EIPA), and NBS was inhibited with HEPES buffer. During anoxia in bicarbonate buffer, cells developed acidosis and intracellular Na and Ca (Nai and Cai, respectively) overload. During reoxygenation cells underwent hypercontracture (44.0 ± 4.1% of the preanoxic length). During anoxia in bicarbonate buffer, inhibition of NHE had no effect on changes in intracellular pH (pHi), Nai, and Cai, but it significantly reduced the reoxygenation-induced hypercontracture (HOE: 61.0 ± 1.4%, EIPA: 68.2 ± 1.8%). The sole inhibition of NBS during anoxia was not protective. We conclude that inhibition of NHE during anoxia protects cardiomyocytes against reoxygenation injury independently of cytosolic acidification and Cai overload.

hypercontracture; pH control; calcium; sodium-hydrogen exchanger


This article has been cited by other articles:


Home page
Cardiovasc ResHome page
S. C. Peters and H. M. Piper
Reoxygenation-induced Ca2+ rise is mediated via Ca2+ influx and Ca2+ release from the endoplasmic reticulum in cardiac endothelial cells
Cardiovasc Res, January 1, 2007; 73(1): 164 - 171.
[Abstract] [Full Text] [PDF]


Home page
Cardiovasc ResHome page
Y. Abdallah, A. Gkatzoflia, H. Pieper, E. Zoga, S. Walther, S. Kasseckert, M. Schafer, K.D. Schluter, H.M. Piper, and C. Schafer
Mechanism of cGMP-mediated protection in a cellular model of myocardial reperfusion injury
Cardiovasc Res, April 1, 2005; 66(1): 123 - 131.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Heart Circ. Physiol.Home page
M. Ten Hove, M. G. J. Nederhoff, and C. J. A. Van Echteld
Relative contributions of Na+/H+ exchange and Na+/HCO3- cotransport to ischemic Nai+ overload in isolated rat hearts
Am J Physiol Heart Circ Physiol, January 1, 2005; 288(1): H287 - H292.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Heart Circ. Physiol.Home page
M. Ruiz-Meana, D. Garcia-Dorado, P. Pina, J. Inserte, L. Agullo, and J. Soler-Soler
Cariporide preserves mitochondrial proton gradient and delays ATP depletion in cardiomyocytes during ischemic conditions
Am J Physiol Heart Circ Physiol, August 7, 2003; 285(3): H999 - H1006.
[Abstract] [Full Text] [PDF]


Home page
Cardiovasc ResHome page
D. G Allen and X.-H. Xiao
Role of the cardiac Na+/H+ exchanger during ischemia and reperfusion
Cardiovasc Res, March 15, 2003; 57(4): 934 - 941.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online