AJP - Heart Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 279: H2743-H2751, 2000;
0363-6135/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (34)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kleschyov, A. L.
Right arrow Articles by Stoclet, J.-C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kleschyov, A. L.
Right arrow Articles by Stoclet, J.-C.
Vol. 279, Issue 6, H2743-H2751, December 2000

Adventitia-derived nitric oxide in rat aortas exposed to endotoxin: cell origin and functional consequences

Andrei L. Kleschyov1, Bernard Muller1, Thérèse Keravis1, Marie-Elisabeth Stoeckel2, and Jean-Claude Stoclet1

1 Pharmacologie et Physico-Chimie des Interactions Cellulaires et Moléculaires, Unité Mixte de Recherche 7034, and 2 Institut de Physiologie et Chimie Biologique, Unité Mixte de Recherche 7519, Centre National de la Recherche Scientifique, Université Louis Pasteur de Strasbourg, 67401 Illkirch, France

The role of adventitial cells in bacterial lipopolysaccharide (LPS)-induced vascular nitric oxide (NO) overproduction has been largely ignored. In rat aortas exposed to LPS in vitro or in vivo, it was found that adventitia contained the major part of NO synthase (NOS)-2 protein (Western blot and immunohistochemistry) and generated the largest amount of NO (electron paramagnetic resonance spin trapping). NOS-2 immunoreactive cells were mainly resident macrophages at an early stage (5 h, in vitro or in vivo) and fibroblasts at a later stage (20 h, in vitro). Adventitial NOS-2 activity largely accounted for 1) the relaxing effect of L-arginine in rings exposed to LPS in vivo, 2) generation of an "NO store" revealed by N-acetylcysteine-induced relaxation, and 3) formation of protein-bound dinitrosyl iron complexes in the medial layer of aortic rings exposed to LPS in vitro. In conclusion, the adventitia is a powerful source of NO triggered by LPS in the rat aorta. This novel source of NO has an important impact on smooth muscle function and might be implicated in various inflammatory diseases.

adventitial fibroblasts; adventitial macrophages; dinitrosyl iron complexes; electron paramagnetic resonance spin trapping


This article has been cited by other articles:


Home page
 Cardiovasc ResHome page
F. A. Auger, P. D'Orleans-Juste, and L. Germain
Adventitia contribution to vascular contraction: Hints provided by tissue-engineered substitutes
Cardiovasc Res, September 1, 2007; 75(4): 669 - 678.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Lakshminrusimha, J. A. Russell, S. F. Gugino, R. M. Ryan, B. Mathew, L. C. Nielsen, and F. C. Morin III
Adjacent bronchus attenuates pulmonary arterial contractility
Am J Physiol Lung Cell Mol Physiol, September 1, 2006; 291(3): L473 - L478.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. Eguchi, L. V. d'Uscio, C. Wambi, D. Weiler, I. Kovesdi, T. O'Brien, and Z. S. Katusic
Inhibitory effect of recombinant iNOS gene expression on vasomotor function of canine basilar artery
Am J Physiol Heart Circ Physiol, December 1, 2002; 283(6): H2560 - H2566.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C. A. J. Schulze-Bauer, P. Regitnig, and G. A. Holzapfel
Mechanics of the human femoral adventitia including the high-pressure response
Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H2427 - H2440.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C. A. J. Schulze-Bauer, P. Regitnig, and G. A. Holzapfel
Mechanics of the human femoral adventitia including the high-pressure response
Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H2427 - H2440.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online