AJP - Heart AJP: Heart and Circulatory Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 279: H3076-H3088, 2000;
0363-6135/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (12)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Suadicani, S. O.
Right arrow Articles by Spray, D. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Suadicani, S. O.
Right arrow Articles by Spray, D. C.
Vol. 279, Issue 6, H3076-H3088, December 2000

Slow intercellular Ca2+ signaling in wild-type and Cx43-null neonatal mouse cardiac myocytes

Sylvia O. Suadicani1,3, Monique J. Vink1, and David C. Spray1,2

1 Department of Neuroscience, 2 Division of Cardiology, Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461; and 3 University São Judas Tadeu, São Paulo 03166, Brazil

Focal mechanical stimulation of single neonatal mouse cardiac myocytes in culture induced intercellular Ca2+ waves that propagated with mean velocities of ~14 µm/s, reaching ~80% of the cells in the field. Deletion of connexin43 (Cx43), the main cardiac gap junction channel protein, did not prevent communication of mechanically induced Ca2+ waves, although the velocity and number of cells communicated by the Ca2+ signal were significantly reduced. Similar effects were observed in wild-type cardiac myocytes treated with heptanol, a gap junction channel blocker. Fewer cells were involved in intercellular Ca2+ signaling in both wild-type and Cx43-null cultures in the presence of suramin, a P2-receptor blocker; blockage was more effective in Cx43-null than in wild-type cells. Thus gap junction channels provide the main pathway for communication of slow intercellular Ca2+ signals in wild-type neonatal mouse cardiac myocytes. Activation of P2-receptors induced by ATP release contributes a secondary, extracellular pathway for transmission of Ca2+ signals. The importance of such ATP-mediated Ca2+ signaling would be expected to be enhanced under ischemic conditions, when release of ATP is increased and gap junction channels conductance is significantly reduced.

calcium waves; connexin; gap junctions; purinergic receptors; intercellular communication


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
G. R. Dubyak
Both sides now: multiple interactions of ATP with pannexin-1 hemichannels. Focus on "A permeant regulating its permeation pore: inhibition of pannexin 1 channels by ATP"
Am J Physiol Cell Physiol, February 1, 2009; 296(2): C235 - C241.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
F. Hanner, J. von Maltzahn, S. Maxeiner, I. Toma, A. Sipos, O. Kruger, K. Willecke, and J. Peti-Peterdi
Connexin45 is expressed in the juxtaglomerular apparatus and is involved in the regulation of renin secretion and blood pressure
Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2008; 295(2): R371 - R380.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
D. P. Slovut, S. H. Mehta, A. M. Dorrance, F. C. Brosius, S. W. Watts, and R.C. Webb
Increased vascular sensitivity and connexin43 expression after sympathetic denervation
Cardiovasc Res, May 1, 2004; 62(2): 388 - 396.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online