AJP - Heart Calcium Transients and Cell-Sarcomere
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 279: H3101-H3112, 2000;
0363-6135/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (46)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Esposito, G.
Right arrow Articles by Rockman, H. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Esposito, G.
Right arrow Articles by Rockman, H. A.
Vol. 279, Issue 6, H3101-H3112, December 2000

SPECIAL COMMUNICATION
Cellular and functional defects in a mouse model of heart failure

Giovanni Esposito*,1, L. F. Santana*,2,3,4, Keith Dilly*,3, Jader Dos Santos Cruz3,5, Lan Mao1, W. J. Lederer2,3, and Howard A. Rockman1

1 Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710; 2 Medical Biotechnology Center, University of Maryland Biotechnology Institute, and 3 Department of Physiology, University of Maryland School of Medicine, Baltimore, Maryland 21201; 4 Institute of Neurobiology, University of Puerto Rico, San Juan, Puerto Rico 00901; and 5 Departamento de Bioquimica e Imunologia, Laboratorio de Membranas Excitaveis, Universidade Federal de Minas Gerais, Minas Gerais, Brazil

Heart failure and dilated cardiomyopathy develop in mice that lack the muscle LIM protein (MLP) gene (MLP-/-). The character and extent of the heart failure that occurs in MLP-/- mice were investigated using echocardiography and in vivo pressure-volume (P-V) loop measurements. P-V loop data were obtained with a new method for mice (sonomicrometry) using two pairs of orthogonal piezoelectric crystals implanted in the endocardial wall. Sonomicrometry revealed right-shifted P-V loops in MLP-/- mice, depressed systolic contractility, and additional evidence of heart failure. Cellular changes in MLP-/- mice were examined in isolated single cells using patch-clamp and confocal Ca2+ concentration ([Ca2+]) imaging techniques. This cellular investigation revealed unchanged Ca2+ currents and Ca2+ spark characteristics but decreased intracellular [Ca2+] transients and contractile responses and a defect in excitation-contraction coupling. Normal cellular and whole heart function was restored in MLP-/- mice that express a cardiac-targeted transgene, which blocks the function of beta -adrenergic receptor (beta -AR) kinase-1 (beta ARK1). These data suggest that, despite the persistent stimulus to develop heart failure in MLP-/- mice (i.e., loss of the structural protein MLP), downregulation and desensitization of the beta -ARs may play a pivotal role in the pathogenesis. Furthermore, this work suggests that the inhibition of beta ARK1 action may prove an effective therapy for heart failure.

contractility; beta -adrenergic receptor; excitation-contraction coupling; calcium signaling; transgenic; beta -adrenergic receptor kinase

* G. Esposito, L. F. Santana, and K. Dilly contributed equally to this work.




This article has been cited by other articles:


Home page
 Lab AnimHome page
J. Stypmann, M. A Engelen, C. Troatz, M. Rothenburger, L. Eckardt, and K. Tiemann
Echocardiographic assessment of global left ventricular function in mice
Lab Anim, April 1, 2009; 43(2): 127 - 137.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
B. O'Rourke
The Ins and Outs of Calcium in Heart Failure
Circ. Res., June 6, 2008; 102(11): 1301 - 1303.
[Full Text] [PDF]

Home page
 RadiologyHome page
B. B. Chin, S. D. Metzler, A. Lemaire, A. Curcio, S. Vemulapalli, K. L. Greer, N. A. Petry, T. G. Turkington, R. E. Coleman, H. Rockman, et al.
Left Ventricular Functional Assessment in Mice: Feasibility of High Spatial and Temporal Resolution ECG-gated Blood Pool SPECT
Radiology, November 1, 2007; 245(2): 440 - 448.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
T. Guo, X. Ai, T. R. Shannon, S. M. Pogwizd, and D. M. Bers
Intra Sarcoplasmic Reticulum Free [Ca2+] and Buffering in Arrhythmogenic Failing Rabbit Heart
Circ. Res., October 12, 2007; 101(8): 802 - 810.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
P. N. Costandi, L. R. Frank, A. D. McCulloch, and J. H. Omens
Role of diastolic properties in the transition to failure in a mouse model of the cardiac dilatation
Am J Physiol Heart Circ Physiol, December 1, 2006; 291(6): H2971 - H2979.
[Abstract] [Full Text] [PDF]

Home page
 J Am Coll CardiolHome page
M. Hoshijima, R. Knoll, M. Pashmforoush, and K. R. Chien
Reversal of Calcium Cycling Defects in Advanced Heart Failure: Toward Molecular Therapy
J. Am. Coll. Cardiol., October 27, 2006; 48(9_Suppl_A): A15 - A23.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. Reyes, M. E. Steinhelper, J. A. Alvarez, D. Escobedo, J. Pearce, J. W. Valvano, B. H. Pollock, C.-L. Wei, A. Kottam, D. Altman, et al.
Impact of physiological variables and genetic background on myocardial frequency-resistivity relations in the intact beating murine heart
Am J Physiol Heart Circ Physiol, October 1, 2006; 291(4): H1659 - H1669.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
J. R. Wilding, F. Joubert, C. de Araujo, D. Fortin, M. Novotova, V. Veksler, and R. Ventura-Clapier
Altered energy transfer from mitochondria to sarcoplasmic reticulum after cytoarchitectural perturbations in mice hearts
J. Physiol., August 15, 2006; 575(1): 191 - 200.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
P. S. Petkova-Kirova, E. Gursoy, H. Mehdi, C. F. McTiernan, B. London, and G. Salama
Electrical remodeling of cardiac myocytes from mice with heart failure due to the overexpression of tumor necrosis factor-{alpha}
Am J Physiol Heart Circ Physiol, May 1, 2006; 290(5): H2098 - H2107.
[Abstract] [Full Text] [PDF]

Home page
 Eur Heart JHome page
S. Watanabe, J. Shite, H. Takaoka, T. Shinke, Y. Imuro, T. Ozawa, H. Otake, D. Matsumoto, D. Ogasawara, O. L. Paredes, et al.
Myocardial stiffness is an important determinant of the plasma brain natriuretic peptide concentration in patients with both diastolic and systolic heart failure
Eur. Heart J., April 1, 2006; 27(7): 832 - 838.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
P. Penela, C. Murga, C. Ribas, A. S. Tutor, S. Peregrin, and F. Mayor Jr.
Mechanisms of regulation of G protein-coupled receptor kinases (GRKs) and cardiovascular disease
Cardiovasc Res, January 1, 2006; 69(1): 46 - 56.
[Abstract] [Full Text] [PDF]

Home page
 J Am Coll CardiolHome page
C. Perrino, S. V. Naga Prasad, M. Patel, M. J. Wolf, and H. A. Rockman
Targeted Inhibition of {beta}-Adrenergic Receptor Kinase-1-Associated Phosphoinositide-3 Kinase Activity Preserves {beta}-Adrenergic Receptor Signaling and Prolongs Survival in Heart Failure Induced by Calsequestrin Overexpression
J. Am. Coll. Cardiol., June 7, 2005; 45(11): 1862 - 1870.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
B. J.C. van den Bosch, C. M.M. van den Burg, K. Schoonderwoerd, P. J. Lindsey, H. R. Scholte, R. F.M. de Coo, E. van Rooij, H. A. Rockman, P. A. Doevendans, and H. J.M. Smeets
Regional absence of mitochondria causing energy depletion in the myocardium of muscle LIM protein knockout mice
Cardiovasc Res, February 1, 2005; 65(2): 411 - 418.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. Skavdahl, C. Steenbergen, J. Clark, P. Myers, T. Demianenko, L. Mao, H. A. Rockman, K. S. Korach, and E. Murphy
Estrogen receptor-{beta} mediates male-female differences in the development of pressure overload hypertrophy
Am J Physiol Heart Circ Physiol, February 1, 2005; 288(2): H469 - H476.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
H. Kan, D. Birkle, A. C. Jain, C. Failinger, S. Xie, and M. S. Finkel
p38 MAP kinase inhibitor reverses stress-induced cardiac myocyte dysfunction
J Appl Physiol, January 1, 2005; 98(1): 77 - 82.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. L. Bale, M. Hoshijima, Y. Gu, N. Dalton, K. R. Anderson, K.-F. Lee, J. Rivier, K. R. Chien, W. W. Vale, and K. L. Peterson
The cardiovascular physiologic actions of urocortin II: Acute effects in murine heart failure
PNAS, March 9, 2004; 101(10): 3697 - 3702.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
H. Kan, Z. Xie, and M. S. Finkel
p38 MAP kinase-mediated negative inotropic effect of HIV gp120 on cardiac myocytes
Am J Physiol Cell Physiol, January 1, 2004; 286(1): C1 - C7.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. Zheng, K. Dilly, J. Dos Santos Cruz, M. Li, Y. Gu, J. A. Ursitti, J. Chen, J. Ross Jr., K. R. Chien, J. W. Lederer, et al.
Sarcoplasmic reticulum calcium defect in Ras-induced hypertrophic cardiomyopathy heart
Am J Physiol Heart Circ Physiol, January 1, 2004; 286(1): H424 - H433.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. R. Keys, E. A. Greene, C. J. Cooper, S. V. Naga Prasad, H. A. Rockman, and W. J. Koch
Cardiac hypertrophy and altered {beta}-adrenergic signaling in transgenic mice that express the amino terminus of {beta}-ARK1
Am J Physiol Heart Circ Physiol, November 1, 2003; 285(5): H2201 - H2211.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
J. G. Dobson, J. Fray, J. L. Leonard, and R. E. Pratt
Molecular mechanisms of reduced {beta}-adrenergic signaling in the aged heart as revealed by genomic profiling
Physiol Genomics, October 17, 2003; 15(2): 142 - 147.
[Abstract] [Full Text] [PDF]

Home page
 Eur Heart JHome page
D. J. Lips, L. J. deWindt, D. J.W. van Kraaij, and P. A. Doevendans
Molecular determinants of myocardial hypertrophy and failure: alternative pathways for beneficial and maladaptive hypertrophy
Eur. Heart J., May 2, 2003; 24(10): 883 - 896.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
L F Santana, E G Chase, V S Votaw, M. T Nelson, and R Greven
Functional coupling of calcineurin and protein kinase A in mouse ventricular myocytes
J. Physiol., October 1, 2002; 544(1): 57 - 69.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J. N. Lorenz
A practical guide to evaluating cardiovascular, renal, and pulmonary function in mice
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2002; 282(6): R1565 - R1582.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. M. Roth, J. S. Swaney, N. D. Dalton, E. A. Gilpin, and J. Ross Jr.
Impact of anesthesia on cardiac function during echocardiography in mice
Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H2134 - H2140.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
H. Takaoka, G. Esposito, L. Mao, H. Suga, and H. A. Rockman
Heart size-independent analysis of myocardial function in murine pressure overload hypertrophy
Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H2190 - H2197.
[Abstract] [Full Text] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
M. HOSHIJIMA, M. PASHMFOROUSH, R. KNOLL, and K.R. CHIEN
The MLP Family of Cytoskeletal Z Disc Proteins and Dilated Cardiomyopathy: A Stress Pathway Model for Heart Failure Progression
Cold Spring Harb Symp Quant Biol, January 1, 2002; 67(0): 399 - 408.
[Abstract] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
S.V. NAGA PRASAD, J. NIENABER, and H.A. ROCKMAN
G-Protein-coupled Receptor Function in Heart Failure
Cold Spring Harb Symp Quant Biol, January 1, 2002; 67(0): 439 - 444.
[Abstract] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C. Y. T. Hart, J. C. Burnett Jr., and M. M. Redfield
Effects of avertin versus xylazine-ketamine anesthesia on cardiac function in normal mice
Am J Physiol Heart Circ Physiol, November 1, 2001; 281(5): H1938 - H1945.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
H. Takaoka, G. Esposito, L. Mao, H. Suga, and H. A. Rockman
Heart size-independent analysis of myocardial function in murine pressure overload hypertrophy
Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H2190 - H2197.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online