AJP - Heart Watch the video to see how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 279: H3118-H3123, 2000;
0363-6135/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (9)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gustafson, L. A.
Right arrow Articles by Van Beek, J. H. G. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gustafson, L. A.
Right arrow Articles by Van Beek, J. H. G. M.
Vol. 279, Issue 6, H3118-H3123, December 2000

SPECIAL COMMUNICATION
Measurement of the activation time of oxidative phosphorylation in isolated mouse hearts

Lori A. Gustafson and Johannes H. G. M. Van Beek

Laboratory for Physiology, Institute for Cardiovascular Research, Vrije Universiteit, 1105 AZ Amsterdam, The Netherlands

The purpose of this study was to develop a technique for determination of the dynamic regulation of oxidative myocardial metabolism in the mouse. The response time of myocardial oxygen consumption (MVO2) to a step in heart rate was determined in Langendorff-perfused mouse hearts. We examined the effect of glucose-only perfusate and glucose combined with 1, 3, or 6 mM pyruvate. Left ventricular systolic pressure (LVSP) decreased, yet the rate-pressure product (RPP) and MVO2 increased with upward steps in heart rate. Pyruvate increased LVSP, RPP, and MVO2 at the lower concentrations; however, when 6 mM pyruvate was added, LVSP and RPP became depressed while MVO2 remained elevated. The mean response time of oxygen consumption to a step in heart rate from 270 to 350 beats/min was 9.8 s (n = 7) in the glucose-only perfused hearts. Perfusion with glucose plus 6 mM pyruvate decreased the response time to 5.3 s. These results are similar to those found in the rabbit heart and lay the groundwork for further examination of the dynamic regulation of oxidative myocardial metabolism in genetically altered mice. We concluded that the activation time of oxidative phosphorylation in the mouse is similar to that in larger species, despite the high mitochondrial content and natural heart rate of the mouse.

energy metabolism; pyruvate


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
Z. Z. Kojic, U. Flogel, J. Schrader, and U. K. M. Decking
Endothelial NO formation does not control myocardial O2 consumption in mouse heart
Am J Physiol Heart Circ Physiol, June 5, 2003; 285(1): H392 - H397.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
L. A. Gustafson and J. H. G. M. Van Beek
Activation time of myocardial oxidative phosphorylation in creatine kinase and adenylate kinase knockout mice
Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H2259 - H2264.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
L. A. Gustafson and J. H. G. M. Van Beek
Activation time of myocardial oxidative phosphorylation in creatine kinase and adenylate kinase knockout mice
Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H2259 - H2264.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online