AJP - Heart BIOPAC complete lab solutions
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 279: H3138-H3143, 2000;
0363-6135/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (19)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kan, H.
Right arrow Articles by Finkel, M. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kan, H.
Right arrow Articles by Finkel, M. S.
Vol. 279, Issue 6, H3138-H3143, December 2000

RAPID COMMUNICATION
HIV gp120 enhances NO production by cardiac myocytes through p38 MAP kinase-mediated NF-kappa B activation

Hong Kan1, Zirong Xie1, and Mitchell S. Finkel1,2,3

Departments of 1 Medicine and 2 Pharmacology, Robert C. Byrd Health Sciences Center, West Virginia University School of Medicine, Morgantown 26506-9157; and 3 Louis A. Johnson Veterans Affairs Medical Center, Clarksburg, West Virginia 26301

Human immunodeficiency virus (HIV) infection is associated with a surprisingly high frequency of myocardial dysfunction. Potential mechanisms include direct effects of HIV, indirect effects mediated by cytokines, or a combination. We have previously reported that interleukin-1beta (IL-1beta ) (500 U/ml) alone induced nitric oxide (NO) production by neonatal rat cardiac myocytes (CM). Effects of the HIV-1 envelope, glycoprotein120 (gp120), on inducible NO synthase (iNOS) in CM have not been previously reported. Unlike IL-1beta , recombinant HIV-gp120 (1 µg/ml) alone failed to enhance NO production in CM (0.5 ± 0.4 vs. 0.4 ± 0.5 µmol/1.25 × 105 cells/48 h, gp120 vs. control, respectively; n = 12, P = not significant). However, the addition of gp120 to IL-1beta significantly enhanced iNOS mRNA expression (70 ± 1.5 vs. 26 ± 2.4 optical units, IL-1beta  + gp120 vs. IL-1beta , respectively; n = 3), iNOS protein synthesis (42 ± 1.4 vs. 18 ± 0.8 optical units, IL-1beta  + gp120 vs. IL-1beta , respectively; n = 3), and NO production (NO2-) (6.6 ± 0.6 vs. 4.1 ± 0.8 µmol/1.25 × 105 cells/48 h, IL-1beta  + gp120 vs. IL-1beta , respectively; n = 12, P <=  0.5). HIV-gp120 enhancement of IL-1beta -induced NO2- production was blocked by 10 µM of SB-203580 (SB), a selective p38 protein kinase inhibitor (3.6 ± 0.2 vs. 6.6 ± 0.6 µmol/1.25 × 105 cells/48 h, IL-1beta  + gp120 + SB vs. IL-1beta  + gp120, respectively; n = 12, P <=  0.5). HIV-gp120-enhanced p38 protein kinase activity was associated with an increase in IL-1beta -stimulated NF-kappa B activity (184 ± 12.7 vs. 92 ± 10.7 optical units, IL-1beta  + gp120 vs. IL-1beta , respectively; n = 3). None of these effects was seen with another recombinant HIV-1 protein, Tat. Thus HIV-gp120 enhancement of IL-1beta -induced NO production is associated with p38-mediated activation of NF-kappa B. Direct effects of HIV-gp120 on CM may provide a previously unrecognized mechanism contributing to HIV cardiomyopathy.

cytokines; heart; cell signaling; human immunodeficiency virus; nitric oxide


This article has been cited by other articles:


Home page
 Am. J. Pathol.Home page
E. A. Eugenin, S. Morgello, M. E. Klotman, A. Mosoian, P. A. Lento, J. W. Berman, and A. D. Schecter
Human Immunodeficiency Virus (HIV) Infects Human Arterial Smooth Muscle Cells in Vivo and in Vitro: Implications for the Pathogenesis of HIV-Mediated Vascular Disease
Am. J. Pathol., April 1, 2008; 172(4): 1100 - 1111.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
C. Lee, B. Tomkowicz, B. D. Freedman, and R. G. Collman
HIV-1 gp120-induced TNF-{alpha} production by primary human macrophages is mediated by phosphatidylinositol-3 (PI-3) kinase and mitogen-activated protein (MAP) kinase pathways
J. Leukoc. Biol., October 1, 2005; 78(4): 1016 - 1023.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
K. Liu, D. S. Chi, C. Li, H. K. Hall, D. M. Milhorn, and G. Krishnaswamy
HIV-1 Tat protein-induced VCAM-1 expression in human pulmonary artery endothelial cells and its signaling
Am J Physiol Lung Cell Mol Physiol, August 1, 2005; 289(2): L252 - L260.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
D. Hargett, T. McLean, and S. L. Bachenheimer
Herpes Simplex Virus ICP27 Activation of Stress Kinases JNK and p38
J. Virol., July 1, 2005; 79(13): 8348 - 8360.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
H. Kan, Z. Xie, and M. S. Finkel
p38 MAP kinase-mediated negative inotropic effect of HIV gp120 on cardiac myocytes
Am J Physiol Cell Physiol, January 1, 2004; 286(1): C1 - C7.
[Abstract] [Full Text] [PDF]

Home page
 J Am Coll CardiolHome page
M. Qing, K. Schumacher, R. Heise, M. Woltje, J. F. Vazquez-Jimenez, T. Richter, M. Arranda-Carrero, J. Hess, G.o. von Bernuth, and M.-C. Seghaye
Intramyocardial synthesis of pro- and anti-inflammatory cytokines in infants with congenital cardiac defects
J. Am. Coll. Cardiol., June 18, 2003; 41(12): 2266 - 2274.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online