| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Physiology, College of Medicine, University of South Alabama, Mobile, Alabama 36688; and 2 Department of Nephrology, University Hospital of Lund, S-221 85 Lund, Sweden
This study was undertaken to evaluate the role of
transcytosis as a bulk transfer mechanism for the passage of albumin
from blood to tissue. Isolated rat lungs were continuously weighed and
perfused with an albumin-serum buffer solution under strictly controlled hemodynamic conditions, which allowed measurements of
microvascular pressure and of the capillary filtration coefficient (LpS). With the use of a tissue
uptake technique, it was possible to determine lung albumin
clearance under isogravimetric conditions (Cliso), or at elevated filtration rates, to obtain an
"apparent albumin reflection coefficient" (
alb).
Experiments were performed during control and after reducing lung
temperature from 35° to 22°C and after infusions of the
transcytosis inhibitors N-ethylmaleimide (NEM) or filipin.
Cooling moderately increased vascular resistance and reduced
LpS and Cliso largely in
proportion to the induced increases in viscosity. At 35°C, NEM (0.13 mM) caused a marked increase in Lp5 and in
Cl150 and also caused a reduction in alb. Furthermore, Cliso increased for the highest dose of
filipin tested (1.8 µg/ml). The demonstrated relative cooling
insensitivity of the transfer of albumin across the endothelium in rat
lungs does not support the contention of transcytosis of proteins
across the endothelium. Furthermore, neither NEM nor filipin inhibited lung microvascular albumin transport, but actually increased lung endothelial permeability.
capillary permeability; macromolecules; transcytosis; albumin clearance
This article has been cited by other articles:
|
|
 |
|
  B.-I. Rosengren, A. Rippe, C. Rippe, D. Venturoli, K. Sward, and B. Rippe Transvascular protein transport in mice lacking endothelial caveolae Am J Physiol Heart Circ Physiol, September 1, 2006; 291(3): H1371 - H1377. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Predescu, S. M. Vogel, and A. B. Malik Functional and morphological studies of protein transcytosis in continuous endothelia Am J Physiol Lung Cell Mol Physiol, November 1, 2004; 287(5): L895 - L901. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Heckel, R. Kiefmann, M. Dorger, M. Stoeckelhuber, and A. E. Goetz Colloidal gold particles as a new in vivo marker of early acute lung injury Am J Physiol Lung Cell Mol Physiol, October 1, 2004; 287(4): L867 - L878. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. LAI-FOOK Pleural Mechanics and Fluid Exchange Physiol Rev, April 1, 2004; 84(2): 385 - 410. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Parker and M. I. Townsley Evaluation of lung injury in rats and mice Am J Physiol Lung Cell Mol Physiol, February 1, 2004; 286(2): L231 - L246. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Moe, R. L. Conhaim, and S. J. Lai-Fook Interstitial albumin concentration measured during growth of perivascular cuffs in liquid-filled rabbit lung J Appl Physiol, January 1, 2004; 96(1): 283 - 292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Bodega, L. Zocchi, and E. Agostoni Albumin transcytosis in mesothelium Am J Physiol Lung Cell Mol Physiol, January 1, 2002; 282(1): L3 - L11. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Vogel, R. D. Minshall, M. Pilipovic', C. Tiruppathi, and A. B. Malik Albumin uptake and transcytosis in endothelial cells in vivo induced by albumin-binding protein Am J Physiol Lung Cell Mol Physiol, December 1, 2001; 281(6): L1512 - L1522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Carlsson, B.-I. Rosengren, and B. Rippe Transcytosis inhibitor N-ethylmaleimide increases microvascular permeability in rat muscle Am J Physiol Heart Circ Physiol, October 1, 2001; 281(4): H1728 - H1733. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
