| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
/
reduces ischemic injury and
does not block protective effects of preconditioning
1 Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, 27709; 2 University of North Carolina, Chapel Hill, 27516; and 3 Duke University Medical Center, Durham, North Carolina 27710
We examined the
effect of inhibition of p38 mitogen-activated protein kinase (MAPK)
/
during ischemia and preconditioning by using the inhibitor
SB-202190. Isolated rat hearts were perfused with Krebs-Henseleit
buffer, while left ventricular developed pressure (LVDP) and
31P nuclear magnetic resonance spectra were acquired
continuously. After 20 min of ischemia and 25 min of reperfusion,
recovery of LVDP in untreated hearts was 32 ± 4%, whereas hearts
treated with SB-202190 5 min before ischemia recovered 59 ± 7%
of their pretreatment LVDP. Preconditioning improved functional
recovery to 65 ± 5%, which was unaffected by SB-202190
treatment, added either throughout the preconditioning protocol
(56 ± 5% recovery) or during the final reperfusion period of
preconditioning (71 ± 11% recovery). Necrosis was assessed after
40 min of ischemia and 2 h of reperfusion using
2,3,5-triphenyltetrazolium chloride (TTC) staining and creatine kinase
release. The untreated group had 54 ± 8% necrotic myocardium, whereas the SB-202190-treated group had 32 ± 7% and the
preconditioned group had 21 ± 4% necrotic tissue by TTC staining.
SB-202190; 31P nuclear magnetic resonance; necrosis; intracellular pH; left ventricular developed pressure
This article has been cited by other articles:
|
|
 |
|
  S. W. Rabkin and M. Y. C. Tsang The action of nitric oxide to enhance cell survival in chick cardiomyocytes is mediated through a cGMP and ERK1/2 pathway while p38 mitogen-activated protein kinase-dependent pathways do not alter cell death Exp Physiol, July 1, 2008; 93(7): 834 - 842. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Murphy and C. Steenbergen Mechanisms Underlying Acute Protection From Cardiac Ischemia-Reperfusion Injury Physiol Rev, April 1, 2008; 88(2): 581 - 609. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Heidbreder, A. Naumann, K. Tempel, P. Dominiak, and A. Dendorfer Remote vs. ischaemic preconditioning: the differential role of mitogen-activated protein kinase pathways Cardiovasc Res, April 1, 2008; 78(1): 108 - 115. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Wang Mitogen-Activated Protein Kinases in Heart Development and Diseases Circulation, September 18, 2007; 116(12): 1413 - 1423. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Vahebi, A. Ota, M. Li, C. M. Warren, P. P. de Tombe, Y. Wang, and R. J. Solaro p38-MAPK Induced Dephosphorylation of {alpha}-Tropomyosin Is Associated With Depression of Myocardial Sarcomeric Tension and ATPase Activity Circ. Res., February 16, 2007; 100(3): 408 - 415. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Okada, H. Otani, Y. Wu, S. Kyoi, C. Enoki, H. Fujiwara, T. Sumida, R. Hattori, and H. Imamura Role of F-actin organization in p38 MAP kinase-mediated apoptosis and necrosis in neonatal rat cardiomyocytes subjected to simulated ischemia and reoxygenation Am J Physiol Heart Circ Physiol, December 1, 2005; 289(6): H2310 - H2318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Kaiser, J. M. Lyons, J. Y. Duffy, C. J. Wagner, K. M. McLean, T. P. O'Neill, J. M. Pearl, and J. D. Molkentin Inhibition of p38 reduces myocardial infarction injury in the mouse but not pig after ischemia-reperfusion Am J Physiol Heart Circ Physiol, December 1, 2005; 289(6): H2747 - H2751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. House, K. Branch, G. Newman, T. Doetschman, and J. E. J. Schultz Cardioprotection induced by cardiac-specific overexpression of fibroblast growth factor-2 is mediated by the MAPK cascade Am J Physiol Heart Circ Physiol, November 1, 2005; 289(5): H2167 - H2175. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Sumida, H. Otani, S. Kyoi, T. Okada, H. Fujiwara, Y. Nakao, M. Kido, and H. Imamura Temporary blockade of contractility during reperfusion elicits a cardioprotective effect of the p38 MAP kinase inhibitor SB-203580 Am J Physiol Heart Circ Physiol, June 1, 2005; 288(6): H2726 - H2734. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Ballard-Croft, G. Kristo, Y. Yoshimura, E. Reid, B. J. Keith, R. M. Mentzer Jr., and R. D. Lasley Acute adenosine preconditioning is mediated by p38 MAPK activation in discrete subcellular compartments Am J Physiol Heart Circ Physiol, March 1, 2005; 288(3): H1359 - H1366. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. See, W. Thomas, K. Way, A. Tzanidis, A. Kompa, D. Lewis, S. Itescu, and H. Krum p38 mitogen-activated protein kinase inhibition improves cardiac function and attenuates left ventricular remodeling following myocardial infarction in the rat J. Am. Coll. Cardiol., October 19, 2004; 44(8): 1679 - 1689. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Uchiyama, H. Otani, T. Okada, T. Uchiyama, H. Ninomiya, M. Kido, H. Imamura, S. Nakao, and K. Shingu Integrated pharmacological preconditioning in combination with adenosine, a mitochondrial KATP channel opener and a nitric oxide donor J. Thorac. Cardiovasc. Surg., July 1, 2003; 126(1): 148 - 159. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. H. Patel, R. M. Fryer, E. R. Gross, R. A. Bundey, A. K. Hsu, M. Isbell, L. O.V. Eusebi, R. V. Jensen, S. R. Gullans, P. A. Insel, et al. 12-Lipoxygenase in Opioid-Induced Delayed Cardioprotection: Gene Array, Mass Spectrometric, and Pharmacological Analyses Circ. Res., April 4, 2003; 92(6): 676 - 682. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Schulz, S. Belosjorow, P. Gres, J. Jansen, M. C Michel, and G. Heusch p38 MAP kinase is a mediator of ischemic preconditioning in pigs Cardiovasc Res, August 15, 2002; 55(3): 690 - 700. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Wei, E. C. Rothstein, L. Fliegel, L. J. Dell'Italia, and P. A. Lucchesi Differential MAP kinase activation and Na+/H+ exchanger phosphorylation by H2O2 in rat cardiac myocytes Am J Physiol Cell Physiol, November 1, 2001; 281(5): C1542 - C1550. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Schulz, M. V Cohen, M. Behrends, J. M Downey, and G. Heusch Signal transduction of ischemic preconditioning Cardiovasc Res, November 1, 2001; 52(2): 181 - 198. [Full Text] [PDF]
|
|
|
|
|
|
R. M. Fryer, H. H. Patel, A. K. Hsu, and G. J. Gross Stress-activated protein kinase phosphorylation during cardioprotection in the ischemic myocardium Am J Physiol Heart Circ Physiol, September 1, 2001; 281(3): H1184 - H1192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yue, M. Krenz, M. V. Cohen, J. M. Downey, and S. D. Critz Menadione mimics the infarct-limiting effect of preconditioning in isolated rat hearts Am J Physiol Heart Circ Physiol, August 1, 2001; 281(2): H590 - H595. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ninomiya, H. Otani, K. Lu, T. Uchiyama, M. Kido, and H. Imamura Enhanced IPC by activation of pertussis toxin-sensitive and -insensitive G protein-coupled purinoceptors Am J Physiol Heart Circ Physiol, May 1, 2002; 282(5): H1933 - H1943. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
