| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Biomedical Engineering, University of Virginia School of Medicine, Charlottesville, Virginia 22908
To study
selectin-independent leukocyte recruitment and the role of
intercellular adhesion molecule-1 (ICAM-1), we generated mice lacking
all three selectins and ICAM-1 (E/P/L/I
/) by bone marrow
transplantation. These mice were viable and appeared healthy under
vivarium conditions, although they showed a 97% reduction in leukocyte
rolling, a 63% reduction in leukocyte firm adhesion, and a 99%
reduction of neutrophil recruitment in a thioglycollate-induced model
of peritonitis at 4 and 24 h. Mononuclear cell recruitment was
almost unaffected. All residual leukocyte rolling and most leukocyte
adhesion in these mice depended on 
4-integrins, but a
small number of leukocytes (6% of wild-type control) still became adherent in the absence of all known rolling mechanisms (E-, P-, L-selectin and 4-integrins). A striking similarity of
leukocyte adhesion efficiency in E/P/L/ and E/P/I/ mice
suggests a pathway in which leukocyte rolling through L-selectin
requires ICAM-1 for adhesion and recruitment. Comparison of our data
with mice lacking individual or other combinations of adhesion
molecules reveal that elimination of more adhesion molecules further
reduces leukocyte recruitment but the effect is less than additive.
neutrophil adhesion; thioglycollate-induced peritonitis; intravital
microscopy; knockout mice; 4-integrins
This article has been cited by other articles:
|
|
 |
|
  A. Kasorn, P. Alcaide, Y. Jia, K. K. Subramanian, B. Sarraj, Y. Li, F. Loison, H. Hattori, L. E. Silberstein, W. F. Luscinskas, et al. Focal Adhesion Kinase Regulates Pathogen-Killing Capability and Life Span of Neutrophils via Mediating Both Adhesion-Dependent and -Independent Cellular Signals J. Immunol., July 15, 2009; 183(2): 1032 - 1043. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Yakubenia, D. Frommhold, D. Scholch, C. C. Hellbusch, C. Korner, B. Petri, C. Jones, U. Ipe, M. G. Bixel, R. Krempien, et al. Leukocyte trafficking in a mouse model for leukocyte adhesion deficiency II/congenital disorder of glycosylation IIc Blood, August 15, 2008; 112(4): 1472 - 1481. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Frommhold, A. Ludwig, M. G. Bixel, A. Zarbock, I. Babushkina, M. Weissinger, S. Cauwenberghs, L. G. Ellies, J. D. Marth, A. G. Beck-Sickinger, et al. Sialyltransferase ST3Gal-IV controls CXCR2-mediated firm leukocyte arrest during inflammation J. Exp. Med., June 9, 2008; 205(6): 1435 - 1446. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Wagner and J. Jenkins Effects of airway distension on leukocyte recruitment in the mouse tracheal microvasculature J Appl Physiol, April 1, 2007; 102(4): 1528 - 1534. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Sumagin and I. H. Sarelius TNF-{alpha} activation of arterioles and venules alters distribution and levels of ICAM-1 and affects leukocyte-endothelial cell interactions Am J Physiol Heart Circ Physiol, November 1, 2006; 291(5): H2116 - H2125. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. E. Hoefer, N. van Royen, J. E. Rectenwald, E. Deindl, J. Hua, M. Jost, S. Grundmann, M. Voskuil, C. K. Ozaki, J. J. Piek, et al. Arteriogenesis Proceeds via ICAM-1/Mac-1- Mediated Mechanisms Circ. Res., May 14, 2004; 94(9): 1179 - 1185. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. G. Ribeiro, D. A. Maria, S. Adriouch, S. Pechberty, W. H. K. Cabrera, J. Morisset, O. M. Ibanez, and M. Seman Convergent alteration of granulopoiesis, chemotactic activity, and neutrophil apoptosis during mouse selection for high acute inflammatory response J. Leukoc. Biol., October 1, 2003; 74(4): 497 - 506. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Salas, M. Shimaoka, S. Chen, C. V. Carman, and T. Springer Transition From Rolling to Firm Adhesion Is Regulated by the Conformation of the I Domain of the Integrin Lymphocyte Function-associated Antigen-1 J. Biol. Chem., December 20, 2002; 277(52): 50255 - 50262. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. H. Wyman, A. J. Bjornsen, D. J. Elzi, C. W. Smith, K. M. England, M. Kelher, and C. C. Silliman A two-insult in vitro model of PMN-mediated pulmonary endothelial damage: requirements for adherence and chemokine release Am J Physiol Cell Physiol, December 1, 2002; 283(6): C1592 - C1603. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. B. Forlow, J. R. Schurr, J. K. Kolls, G. J. Bagby, P. O. Schwarzenberger, and K. Ley Increased granulopoiesis through interleukin-17 and granulocyte colony-stimulating factor in leukocyte adhesion molecule-deficient mice Blood, December 1, 2001; 98(12): 3309 - 3314. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
