AJP - Heart AJP: Renal Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 280: H1215-H1221, 2001;
0363-6135/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (5)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miyakoshi, K.
Right arrow Articles by Yoshimura, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miyakoshi, K.
Right arrow Articles by Yoshimura, Y.
Vol. 280, Issue 3, H1215-H1221, March 2001

Role of leukocytes in uterine hypoperfusion and fetal growth retardation induced by ischemia-reperfusion

Kei Miyakoshi1, Hitoshi Ishimoto1, Osamu Nishimura1, Shinji Tanigaki1, Mamoru Tanaka1, Toyohiko Miyazaki1, Michiya Natori2, and Yasunori Yoshimura1

1 Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo 160-8582; and 2 Department of Clinical Research, National Ohkura Hospital, Tokyo 157-0063, Japan

We investigated leukocyte involvement in uterine hypoperfusion and intrauterine fetal growth retardation (IUGR) induced by ischemia-reperfusion (I/R) in Sprague-Dawley rats. On day 17 of gestation, leukocyte accumulation in the uterus and placenta subjected to 30 min of ischemia, followed by reperfusion, was assessed by measuring myeloperoxidase (MPO) activity. Uterine MPO activity was significantly higher after 1 h of reperfusion than it was before ischemia (P < 0.05), without any increase in placental MPO activity. Immunohistochemical staining showed leukocyte accumulation in the uterus subjected to I/R. The effects of treatment with monoclonal antibodies against CD11a (WT1) and CD18 (WT3) at a dose of 0.8 mg/kg on uterine blood flow and IUGR were investigated. Laser-Doppler flowmetry demonstrated that uterine hypoperfusion at 2 h after ischemia (blood flow, -51.7 ± 1.2%; P < 0.01) was inhibited by WT1 and WT3 treatment. I/R-induced IUGR at full term (P < 0.05 vs. nonischemic horn) was prevented by WT1 and WT3 treatment on day 17. These results indicate that leukocyte accumulation may play an important role in the pathogenesis of uterine hypoperfusion and IUGR induced by I/R in pregnant rats.

uterine blood flow; CD11/CD18 integrin; rat


This article has been cited by other articles:


Home page
 Am. J. Respir. Crit. Care Med.Home page
K. Yoshida, R. Kondo, Q. Wang, and C. M. Doerschuk
Neutrophil Cytoskeletal Rearrangements during Capillary Sequestration in Bacterial Pneumonia in Rats
Am. J. Respir. Crit. Care Med., September 15, 2006; 174(6): 689 - 698.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
K. Minegishi, M. Tanaka, O. Nishimura, S. Tanigaki, K. Miyakoshi, H. Ishimoto, and Y. Yoshimura
Reactive oxygen species mediate leukocyte-endothelium interactions in prostaglandin F2alpha -induced luteolysis in rats
Am J Physiol Endocrinol Metab, December 1, 2002; 283(6): E1308 - E1315.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online