| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
in opioid-initiated
cardioprotection
1 Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226; and 2 Department of Pathology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267
Stimulation of the 
1-opioid receptor confers
cardioprotection to the ischemic myocardium. We examined the
role of protein kinase C (PKC) after -opioid receptor stimulation
with TAN-67 or
D-Ala2-D-Leu5-enkephalin
(DADLE) in a rat model of myocardial infarction induced by a 30-min
coronary artery occlusion and 2-h reperfusion. Infarct size (IS) was
determined by tetrazolium staining and expressed as a percentage of the
area at risk (IS/AAR). Control animals, subjected to ischemia
and reperfusion, had an IS/AAR of 59.9 ± 1.8. DADLE and TAN-67
administered before ischemia significantly reduced IS/AAR
(36.9 ± 3.9 and 36.7 ± 4.7, respectively). The 1-selective opioid antagonist 7-benzylidenenaltrexone
(BNTX) abolished TAN-67-induced cardioprotection (54.4 ± 1.3).
Treatment with the PKC antagonist chelerythrine completely abolished
DADLE- (61.8 ± 3.2) and TAN-67-induced cardioprotection
(55.4 ± 4.0). Similarly, the PKC antagonist GF 109203X completely
abolished TAN-67-induced cardioprotection (54.6 ± 6.6).
Immunofluorescent staining with antibodies directed against specific
PKC isoforms was performed in myocardial biopsies obtained after 15 min
of treatment with saline, chelerythrine, BNTX, or TAN-67 and
chelerythrine or BNTX in the presence of TAN-67. TAN-67 induced the
translocation of PKC-
to the sarcolemma, PKC-
1 to the
nucleus, PKC- to the mitochondria, and PKC-
to the intercalated
disk and mitochondria. PKC translocation was abolished by chelerythrine
and BNTX in TAN-67-treated rats. To more closely examine the role of
these isoforms in cardioprotection, we utilized the PKC- selective
antagonist rottlerin. Rottlerin abolished opioid-induced
cardioprotection (48.9 ± 4.8) and PKC- translocation without
affecting the translocation of PKC-, -1, or -.
These results suggest that PKC- is a key second messenger in the
cardioprotective effects of 1-opioid receptor
stimulation in rats.
preconditioning; protein kinase C; ischemia
This article has been cited by other articles:
|
|
 |
|
  A. J. Zatta, H. Kin, D. Yoshishige, R. Jiang, N. Wang, J. G. Reeves, J. Mykytenko, R. A. Guyton, Z.-Q. Zhao, J. L. Caffrey, et al. Evidence that cardioprotection by postconditioning involves preservation of myocardial opioid content and selective opioid receptor activation Am J Physiol Heart Circ Physiol, March 1, 2008; 294(3): H1444 - H1451. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. B. Gustafsson and R. A. Gottlieb Heart mitochondria: gates of life and death Cardiovasc Res, January 15, 2008; 77(2): 334 - 343. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Bouwman, R. J. P. Musters, B. J. van Beek-Harmsen, J. J. de Lange, R. R. Lamberts, S. A. Loer, and C. Boer Sevoflurane-induced cardioprotection depends on PKC-{alpha} activation via production of reactive oxygen species Br. J. Anaesth., November 1, 2007; 99(5): 639 - 645. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Miura, T. Yano, K. Naitoh, M. Nishihara, T. Miki, M. Tanno, and K. Shimamoto {delta}-Opioid receptor activation before ischemia reduces gap junction permeability in ischemic myocardium by PKC-{varepsilon}-mediated phosphorylation of connexin 43 Am J Physiol Heart Circ Physiol, September 1, 2007; 293(3): H1425 - H1431. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. House, S. J. Melhorn, G. Newman, T. Doetschman, and J. E. J. Schultz The protein kinase C pathway mediates cardioprotection induced by cardiac-specific overexpression of fibroblast growth factor-2 Am J Physiol Heart Circ Physiol, July 1, 2007; 293(1): H354 - H365. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. Coaxum, T. M. Griffin, J. L. Martin, and R. Mestril Influence of PKC-{alpha} overexpression on HSP70 and cardioprotection Am J Physiol Heart Circ Physiol, May 1, 2007; 292(5): H2220 - H2226. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Inagaki, E. Churchill, and D. Mochly-Rosen Epsilon protein kinase C as a potential therapeutic target for the ischemic heart Cardiovasc Res, May 1, 2006; 70(2): 222 - 230. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. Bovill Intravenous Anesthesia for the Patient with Left Ventricular Dysfunction Seminars in Cardiothoracic and Vascular Anesthesia, March 1, 2006; 10(1): 43 - 48. [Abstract] [PDF]
|
|
|
|
 |
|
 D. Weihrauch, J. G. Krolikowski, M. Bienengraeber, J. R. Kersten, D. C. Warltier, and P. S. Pagel Morphine Enhances Isoflurane-Induced Postconditioning Against Myocardial Infarction: The Role of Phosphatidylinositol-3-Kinase and Opioid Receptors in Rabbits Anesth. Analg., October 1, 2005; 101(4): 942 - 949. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Neckar, I. Markova, F. Novak, O. Novakova, O. Szarszoi, B. Ost'adal, and F. Kolar Increased expression and altered subcellular distribution of PKC-{delta} in chronically hypoxic rat myocardium: involvement in cardioprotection Am J Physiol Heart Circ Physiol, April 1, 2005; 288(4): H1566 - H1572. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Cao, L. Liu, and D. M. Van Winkle Met5-enkephalin-induced cardioprotection occurs via transactivation of EGFR and activation of PI3K Am J Physiol Heart Circ Physiol, April 1, 2005; 288(4): H1955 - H1964. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zhang, C. P. Baines, C. Zong, E. M. Cardwell, G. Wang, T. M. Vondriska, and P. Ping Functional proteomic analysis of a three-tier PKC{varepsilon}-Akt-eNOS signaling module in cardiac protection Am J Physiol Heart Circ Physiol, February 1, 2005; 288(2): H954 - H961. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Hassouna, B. M. Matata, and M. Galinanes PKC-{epsilon} is upstream and PKC-{alpha} is downstream of mitoKATP channels in the signal transduction pathway of ischemic preconditioning of human myocardium Am J Physiol Cell Physiol, November 1, 2004; 287(5): C1418 - C1425. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zhang, P. Ping, G.-W. Wang, M. Lu, D. Pantaleon, X.-L. Tang, R. Bolli, and T. M. Vondriska Bmx, a member of the Tec family of nonreceptor tyrosine kinases, is a novel participant in pharmacological cardioprotection Am J Physiol Heart Circ Physiol, November 1, 2004; 287(5): H2364 - H2366. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Okubo, Y. Tanabe, K. Takeda, M. Kitayama, S. Kanemitsu, R. C. Kukreja, and N. Takekoshi Ischemic preconditioning and morphine attenuate myocardial apoptosis and infarction after ischemia-reperfusion in rabbits: role of {delta}-opioid receptor Am J Physiol Heart Circ Physiol, October 1, 2004; 287(4): H1786 - H1791. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Pepe, O. W.V van den Brink, E. G Lakatta, and R.-P. Xiao Cross-talk of opioid peptide receptor and {beta}-adrenergic receptor signalling in the heart Cardiovasc Res, August 15, 2004; 63(3): 414 - 422. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mayr, B. Metzler, Y.-L. Chung, E. McGregor, U. Mayr, H. Troy, Y. Hu, M. Leitges, O. Pachinger, J. R. Griffiths, et al. Ischemic preconditioning exaggerates cardiac damage in PKC-{delta} null mice Am J Physiol Heart Circ Physiol, August 1, 2004; 287(2): H946 - H956. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sun, M. H. Weil, W. Tang, T. Kamohara, and K. Klouche {delta}-Opioid receptor agonist reduces severity of postresuscitation myocardial dysfunction Am J Physiol Heart Circ Physiol, August 1, 2004; 287(2): H969 - H974. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Smith Sonneborn, H. Gottsch, E. Cubin, P. Oeltgen, and P. Thomas Alternative Strategy for Stress Tolerance: Opioids J. Gerontol. A Biol. Sci. Med. Sci., May 1, 2004; 59(5): B433 - B440. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. R. Gross, A. K. Hsu, and G. J. Gross Opioid-Induced Cardioprotection Occurs via Glycogen Synthase Kinase {beta} Inhibition During Reperfusion in Intact Rat Hearts Circ. Res., April 16, 2004; 94(7): 960 - 966. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Cao, L. Liu, and D. M. Van Winkle Activation of {delta}- and {kappa}-opioid receptors by opioid peptides protects cardiomyocytes via KATP channels Am J Physiol Heart Circ Physiol, August 7, 2003; 285(3): H1032 - H1039. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. C. Zhao and R. C. Kukreja Protein kinase C-{delta} mediates adenosine A3 receptor-induced delayed cardioprotection in mouse Am J Physiol Heart Circ Physiol, June 5, 2003; 285(1): H434 - H441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Coles Jr., D. C. Sigg, and P. A. Iaizzo Role of kappa -opioid receptor activation in pharmacological preconditioning of swine Am J Physiol Heart Circ Physiol, June 1, 2003; 284(6): H2091 - H2099. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Shinmura, M. Nagai, K. Tamaki, M. Tani, and R. Bolli COX-2-derived prostacyclin mediates opioid-induced late phase of preconditioning in isolated rat hearts Am J Physiol Heart Circ Physiol, December 1, 2002; 283(6): H2534 - H2543. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Kodani, Y.-T. Xuan, K. Shinmura, H. Takano, X.-L. Tang, and R. Bolli delta -Opioid receptor-induced late preconditioning is mediated by cyclooxygenase-2 in conscious rabbits Am J Physiol Heart Circ Physiol, November 1, 2002; 283(5): H1943 - H1957. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M Fryer, J. A Auchampach, and G. J Gross Therapeutic receptor targets of ischemic preconditioning Cardiovasc Res, August 15, 2002; 55(3): 520 - 525. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kudo, Y. Wang, M. Xu, A. Ayub, and M. Ashraf Adenosine A1 receptor mediates late preconditioning via activation of PKC-delta signaling pathway Am J Physiol Heart Circ Physiol, July 1, 2002; 283(1): H296 - H301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. W. Dickson, R. J. Tubbs, W. A. Porcaro, W. J. Lee, D. J. Blehar, R. E. Carraway, C. E. Darling, and K. Przyklenk Myocardial preconditioning factors evoke mesenteric ischemic tolerance via opioid receptors and KATP channels Am J Physiol Heart Circ Physiol, July 1, 2002; 283(1): H22 - H28. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Sigg, J. A. Coles Jr., P. R. Oeltgen, and P. A. Iaizzo Role of delta -opioid receptor agonists on infarct size reduction in swine Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H1953 - H1960. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Fryer, Y. Wang, A. K. Hsu, H. Nagase, and G. J. Gross Dependence of delta 1-Opioid Receptor-Induced Cardioprotection on a Tyrosine Kinase-Dependent but Not a Src-Dependent Pathway J. Pharmacol. Exp. Ther., November 1, 2001; 299(2): 477 - 482. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G.-Y. Wang, J. J. Zhou, J. Shan, and T.-M. Wong Protein Kinase C-epsilon Is a Trigger of Delayed Cardioprotection against Myocardial Ischemia of kappa -Opioid Receptor Stimulation in Rat Ventricular Myocytes J. Pharmacol. Exp. Ther., November 1, 2001; 299(2): 603 - 610. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Fryer, H. H. Patel, A. K. Hsu, and G. J. Gross Stress-activated protein kinase phosphorylation during cardioprotection in the ischemic myocardium Am J Physiol Heart Circ Physiol, September 1, 2001; 281(3): H1184 - H1192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.-S. Jiang, R. R. Padua, H. Ju, B. W. Doble, Y. Jin, J. Hao, P. A. Cattini, I. M. C. Dixon, and E. Kardami Acute protection of ischemic heart by FGF-2: involvement of FGF-2 receptors and protein kinase C Am J Physiol Heart Circ Physiol, March 1, 2002; 282(3): H1071 - H1080. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
