AJP - Heart AJP: Endocrinology and Metabolism
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 280: H1422-H1426, 2001;
0363-6135/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (12)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hulvershorn, J.
Right arrow Articles by Morgan, K. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hulvershorn, J.
Right arrow Articles by Morgan, K. G.
Vol. 280, Issue 3, H1422-H1426, March 2001

RAPID COMMUNICATION
Calmodulin levels are dynamically regulated in living vascular smooth muscle cells

Justin Hulvershorn1,2, Cynthia Gallant1, C.-L. Albert Wang2,3, Chantal Dessy1, and Kathleen G. Morgan1,2,4

1 Signal Transduction Group, and 2 Muscle Research Group, Boston Biomedical Research Institute, Watertown 02472; 3 Department of Physiology, Tufts University School of Medicine, Boston 02111; and 4 Cardiovascular Division, Beth Israel Deaconess Medical Center, and Harvard Medical School, Boston, Massachusetts 02215

The total unbound calmodulin (i.e., not bound to target proteins) level in living smooth muscle cells from the ferret portal vein was monitored with a low-affinity, calmodulin-binding peptide tagged with an environmentally sensitive fluorophore. GS17C, a previously characterized peptide, from the calmodulin-binding domain of caldesmon was tagged with iodoacetyl nitrobenz-2-oxa-1,3-diazole (NBD) or, as a negative control, with iodoacetylfluorescein isothiocyanate. Increases in NBD-GS17C fluorescence were detected by using confocal microscopy when chemically loaded cells were stimulated with solutions of elevated [K+] or the calcium ionophore 4-bromoA-23187 to elicit increases in intracellular Ca2+ concentration ([Ca2+]i) quantified by fura 2. Increases in peptide fluorescence were detected in response to a phorbol ester in the absence of changes in [Ca2+]i. These changes were blocked by the addition of the calmodulin antagonist calmidazolium. These results suggest that the total unbound intracellular calmodulin levels may be sufficient to regulate the activity of caldesmon and, furthermore, that phosphorylation of protein kinase C substrates may increase the level of available calmodulin in living smooth muscle cells.

caldesmon; fluorescence; calcium


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
R. Eves, B. A. Webb, S. Zhou, and A. S. Mak
Caldesmon is an integral component of podosomes in smooth muscle cells
J. Cell Sci., May 1, 2006; 119(9): 1691 - 1702.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C. Gallant, J. Y. You, Y. Sasaki, Z. Grabarek, and K. G. Morgan
MARCKS is a major PKC-dependent regulator of calmodulin targeting in smooth muscle
J. Cell Sci., August 15, 2005; 118(16): 3595 - 3605.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
K. G. Morgan and S. S. Gangopadhyay
Signal Transduction in Smooth Muscle: Invited Review: Cross-bridge regulation by thin filament-associated proteins
J Appl Physiol, August 1, 2001; 91(2): 953 - 962.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. P. Wilson, C. Sutherland, and M. P. Walsh
Ca2+ Activation of Smooth Muscle Contraction. EVIDENCE FOR THE INVOLVEMENT OF CALMODULIN THAT IS BOUND TO THE TRITON-INSOLUBLE FRACTION EVEN IN THE ABSENCE OF Ca2+
J. Biol. Chem., January 11, 2002; 277(3): 2186 - 2192.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online