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Am J Physiol Heart Circ Physiol 280: H937-H945, 2001;
0363-6135/01 $5.00
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Vol. 280, Issue 3, H937-H945, March 2001

AE anion exchangers in atrial tumor cells

Panos Papageorgiou, Boris E. Shmukler, Alan K. Stuart-Tilley, Lianwei Jiang, and Seth L. Alper

Harvard-Thorndike Institute of Electrophysiology, Cardiovascular Division, Molecular Medicine and Renal Units, Beth Israel Deaconess Medical Center, Boston; and Departments of Medicine and Cell Biology, Harvard Medical School, Boston, Massachusetts 02215

Intracellular pH homeostasis and intracellular Cl- concentration in cardiac myocytes are regulated by anion exchange mechanisms. In physiological extracellular Cl- concentrations, Cl-/HCO<SUB>3</SUB><SUP>−</SUP> exchange promotes intracellular acidification and Cl- loading sensitive to inhibition by stilbene disulfonates. We investigated the expression of AE anion exchangers in the AT-1 mouse atrial tumor cell line. Cultured AT-1 cells exhibited a substantial basal Na+-independent Cl-/HCO<SUB>3</SUB><SUP>−</SUP> (but not Cl-/OH-) exchange activity that was inhibited by DIDS but not by dibenzamidostilbene disulfonic acid (DBDS). AT-1 cell Cl-/HCO<SUB>3</SUB><SUP>−</SUP> activity was stimulated two- to threefold by extracellular ATP and ANG II. AE mRNAs detected by RT-PCR in AT-1 cells included brain AE3 (bAE3), cardiac AE3 (cAE3), AE2a, AE2b, AE2c1, AE2c2, and erythroid AE1 (eAE1), but not kidney AE1 (kAE1). Cultured AT-1 cells expressed AE2, cAE3, and bAE3 polypeptides, which were detected by immunoblot and immunocytochemistry. An AE1-like epitope was detected by immunocytochemistry but not by immunoblot. Both bAE3 and cAE3 were present in intact AT-1 tumors. Cultured AT-1 cells provide a useful system for the study of mediators and regulators of Cl-/HCO<SUB>3</SUB><SUP>−</SUP> exchange activity in an atrial cell type.

AT-1; band 3; AE1; AE3; chloride-bicarbonate exchange; atrium; heart


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