| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom; and 2 Laboratory of Membrane Biochemistry and Biophysics, National Institute on Alcohol Abuse and Alcoholism, Rockville, Maryland 20852
The physiological role of mitochondrial uncoupling proteins (UCPs) in heart and skeletal muscle is unknown, as is whether mitochondrial uncoupling of oxidative phosphorylation by fatty acids occurs in vivo. In this study, we found that UCP2 and UCP3 protein content, determined using Western blotting, was increased by 32 and 48%, respectively, in hyperthyroid rat heart mitochondria. Oligomycin-insensitive respiration rate, a measure of mitochondrial uncoupling, was increased in all mitochondria in the presence of palmitate: 36% in controls and 71 and 100% with 0.8 and 0.9 mM palmitate, respectively, in hyperthyroid rat heart mitochondria. In the isolated working heart, 0.4 mM palmitate significantly lowered cardiac output by 36% and cardiac efficiency by 38% in the hyperthyroid rat heart. Thus increased mitochondrial UCPs in the hyperthyroid rat heart were associated with increased uncoupling and decreased myocardial efficiency in the presence of palmitate. In conclusion, a physiological effect of UCPs on fatty acid oxidation has been found in heart at the mitochondrial and whole organ level.
isolated mitochondria; cardiac efficiency
This article has been cited by other articles:
|
|
 |
|
  M. F. Essop Cardiac metabolic adaptations in response to chronic hypoxia J. Physiol., November 1, 2007; 584(3): 715 - 726. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Bhashyam, P. Parikh, H. Bolukoglu, A. H. Shannon, J. H. Porter, Y.-T. Shen, and R. P. Shannon Aging is associated with myocardial insulin resistance and mitochondrial dysfunction Am J Physiol Heart Circ Physiol, November 1, 2007; 293(5): H3063 - H3071. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Boudina, S. Sena, H. Theobald, X. Sheng, J. J. Wright, X. X. Hu, S. Aziz, J. I. Johnson, H. Bugger, V. G. Zaha, et al. Mitochondrial Energetics in the Heart in Obesity-Related Diabetes: Direct Evidence for Increased Uncoupled Respiration and Activation of Uncoupling Proteins Diabetes, October 1, 2007; 56(10): 2457 - 2466. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ashrafian, M. P. Frenneaux, and L. H. Opie Metabolic Mechanisms in Heart Failure Circulation, July 24, 2007; 116(4): 434 - 448. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. C. Heather, M. A. Cole, C. A. Lygate, R. D. Evans, D. J. Stuckey, A. J. Murray, S. Neubauer, and K. Clarke Fatty acid transporter levels and palmitate oxidation rate correlate with ejection fraction in the infarcted rat heart Cardiovasc Res, December 1, 2006; 72(3): 430 - 437. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Wilding, F. Joubert, C. de Araujo, D. Fortin, M. Novotova, V. Veksler, and R. Ventura-Clapier Altered energy transfer from mitochondria to sarcoplasmic reticulum after cytoarchitectural perturbations in mice hearts J. Physiol., August 15, 2006; 575(1): 191 - 200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O.-J. How, E. Aasum, D. L. Severson, W.Y. A. Chan, M. F. Essop, and T. S. Larsen Increased Myocardial Oxygen Consumption Reduces Cardiac Efficiency in Diabetic Mice Diabetes, February 1, 2006; 55(2): 466 - 473. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Lee, R. Campbell, M. Scheuermann-Freestone, R. Taylor, P. Gunaruwan, L. Williams, H. Ashrafian, J. Horowitz, A. G. Fraser, K. Clarke, et al. Metabolic Modulation With Perhexiline in Chronic Heart Failure: A Randomized, Controlled Trial of Short-Term Use of a Novel Treatment Circulation, November 22, 2005; 112(21): 3280 - 3288. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Faadiel Essop and L. H. Opie Metabolic therapy for heart failure Eur. Heart J., October 2, 2004; 25(20): 1765 - 1768. [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Larkin, B. C. Frank, R. M. Gaspard, I. Duka, H. Gavras, and J. Quackenbush Cardiac transcriptional response to acute and chronic angiotensin II treatments Physiol Genomics, July 8, 2004; 18(2): 152 - 166. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Vincent, B. Bouchard, M. Khairallah, and C. Des Rosiers Differential modulation of citrate synthesis and release by fatty acids in perfused working rat hearts Am J Physiol Heart Circ Physiol, January 1, 2004; 286(1): H257 - H266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. H. Opie Preconditioning and metabolic anti-ischaemic agents Eur. Heart J., October 2, 2003; 24(20): 1854 - 1856. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J Ashton, K. Holmgren, J. Peart, A. R Lankford, G Paul Matherne, S. Grimmond, and J. P Headrick Effects of A1 adenosine receptor overexpression on normoxic and post-ischemic gene expression Cardiovasc Res, March 1, 2003; 57(3): 715 - 726. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Degens, A. J. Gilde, M. Lindhout, P. H. M. Willemsen, G. J. van der Vusse, and M. van Bilsen Functional and metabolic adaptation of the heart to prolonged thyroid hormone treatment Am J Physiol Heart Circ Physiol, January 1, 2003; 284(1): H108 - H115. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
