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Department of Physiology, University of Bergen, Bergen N-5009, Norway
Volume exclusion, i.e., the space not available
for a specific probe, may be dependant on the probe charge. Therefore,
interstitial exclusion was measured for positively and negatively
charged immunoglobulin (IgG) in skin and muscle of rats by using a
continuous infusion method (30). Steady-state
concentration of 125I-labeled IgG 1 (pI = 8.7) and
131I- labeled IgG 4 (pI = 6.6) was maintained by
infusion of tracer for 120-168 h with an implanted osmotic pump.
At the end of the infusion period and before tissue sampling, the rat
was anesthetized and nephrectomized, and 51Cr-labeled EDTA
was injected and allowed 4 h for equilibration to measure
interstitial fluid volume (Vi). Interstitial fluid was
isolated from skin and muscle by using nylon wicks implanted post
mortem. The relative IgG available space was measured as the ratio
between labeled IgG and 51Cr-labeled EDTA wick fluid
equivalent spaces, and relative excluded volume fraction
(Ve/Vi) was calculated as 1 
Va/Vi. Ve/Vi
in hindlimb skin averaged 0.37 ± 0.05 (SE) and 0.65 ± 0.06 (P < 0.01) for IgG 1 and 4, respectively, with
corresponding figures of 0.24 ± 0.05 and 0.51 ± 0.04 (P < 0.01) in hindlimb muscle (n = 9 for both tissues). These experiments suggest that fixed negative
charges, most likely glycosaminoglycans, influence distribution of
macromolecules in the interstitium and therefore affect interstitial
fluid balance.
extracellular fluid volume; extracellular matrix; immunoglobulin G space; bound immunoglobulin G; immunoglobulin G subclasses
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