AJP - Heart Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 280: H1716-H1721, 2001;
0363-6135/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (12)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schlag, M. G.
Right arrow Articles by Potter, R. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schlag, M. G.
Right arrow Articles by Potter, R. F.
Vol. 280, Issue 4, H1716-H1721, April 2001

Role of leukocyte accumulation and oxygen radicals in ischemia-reperfusion-induced injury in skeletal muscle

M. G. Schlag3,4, K. A. Harris1,2, and R. F. Potter1,2,3

1 Lawson Health Research Institute, Departments of 2 Surgery and 3 Medical Biophysics, University of Western Ontario, London, Ontario N6A 4G5, Canada; and 4 Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Vienna A-1090, Austria

The role of leukocytes and nonleukocyte-derived reactive oxygen metabolites (ROMs) in reperfusion-induced skeletal muscle injury was determined. Male rats received 2 h no-flow hindlimb ischemia-reperfusion (I/R, n = 6) or were rendered neutropenic via antineutrophil serum (ANS) before I/R (I/R + ANS, n = 5). Oxygen radicals in the absence of neutrophils were tested by administration of dimethylthiourea (DMTU) (I/R + ANS + DMTU, n = 5). Perfused capillaries (CDper) and rolling (Lr), adherent (La), and extravasated leukocytes (Le) in the extensor digitorum longus muscle were measured every 15 min during 90 min of reperfusion using intravital microscopy. The vital dyes bisbenzimide (BB) and ethidium bromide (EB) provided direct measures of tissue injury (EB/BB). CDper decreased immediately on reperfusion in the I/R and I/R + ANS groups. CDper in the I/R + ANS + DMTU group remained at baseline throughout reperfusion. La increased in the I/R group; however, EB/BB was the same between I/R and I/R + ANS groups. Injury in the I/R + ANS + DMTU group did not differ from other groups >= 60 min, after which EB/BB became significantly lower. Le did not differ between groups and was highly correlated to tissue injury. The results suggest that Le lead to parenchymal injury, and ROMs lead to perfusion deficits during the early reperfusion period after ischemia.

reactive oxygen; dimethylthiourea; neutropenia


This article has been cited by other articles:


Home page
 Ann. Thorac. Surg.Home page
D. A. Bull and J. Maurer
Aprotinin and preservation of myocardial function after ischemia-reperfusion injury
Ann. Thorac. Surg., February 1, 2003; 75(2): S735 - 739.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
N. Stupka and P. M. Tiidus
Effects of ovariectomy and estrogen on ischemia-reperfusion injury in hindlimbs of female rats
J Appl Physiol, October 1, 2001; 91(4): 1828 - 1835.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online