AJP - Heart Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 280: H2342-H2349, 2001;
0363-6135/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (22)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bouayad, A.
Right arrow Articles by Chemtob, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bouayad, A.
Right arrow Articles by Chemtob, S.
Vol. 280, Issue 5, H2342-H2349, May 2001

Characterization of PGE2 receptors in fetal and newborn lamb ductus arteriosus

Asmàa Bouayad1,*, Hiroki Kajino3,*, Nahid Waleh3, Jean-Claude Fouron1, Gregor Andelfinger1, Daya R. Varma2, Amanda Skoll1, Alejandro Vazquez1, Fernand Gobeil Jr1, Ronald I. Clyman3, and Sylvain Chemtob1,2

1 Departments of Cardiology, Pediatrics, and Physiology, Université de Montréal, Quebec H3T 1C5; 2 Departments of Pharmacology and Therapeutics, McGill University, Quebec, Canada H3G 1Y6; and 3 Cardiovascular Research Institute and Department of Pediatrics, University of California San Francisco, San Francisco, California 94143

Although the role of PGE2 in maintaining ductus arteriosus (DA) patency is well established, the specific PGE2 receptor subtype(s) (EP) involved have not been clearly identified. We used late gestation fetal and neonatal lambs to study developmental regulation of EP receptors. In the fetal DA, radioligand binding and RT-PCR assays virtually failed to detect EP1 but detected EP2, EP3D, and EP4 receptors in equivalent proportions. In the newborn lamb, DA total density was one-third of that found in the fetus and only EP2 was detected. Stimulation of EP2 and EP4 increased cAMP formation and was associated with DA relaxation. Though stimulation of EP3 inhibited cAMP formation, it surprisingly relaxed the fetal DA both in vitro and in vivo. This EP3-induced relaxation was specifically diminished by the ATP-sensitive K+ (KATP) channel blocker glibenclamide. In conclusion, PGE2 dilates the late gestation fetal DA through pathways that involve either cAMP (EP2 and EP4) or KATP channels (EP3). The loss of EP3 and EP4 receptors in the newborn DA is consistent with its decreased responsiveness to PGE2.

ductus arteriosus; EP receptors; ATP-sensitive potassium channels.

* A. Bouayad and H. Kajino have equally contributed to this work.




This article has been cited by other articles:


Home page
 PediatricsHome page
D. McCurnin, S. Seidner, L.-Y. Chang, N. Waleh, M. Ikegami, J. Petershack, B. Yoder, L. Giavedoni, K. H. Albertine, M. J. Dahl, et al.
Ibuprofen-Induced Patent Ductus Arteriosus Closure: Physiologic, Histologic, and Biochemical Effects on the Premature Lung
Pediatrics, May 1, 2008; 121(5): 945 - 956.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
R. I. Clyman, N. Waleh, H. Kajino, C. Roman, and F. Mauray
Calcium-dependent and calcium-sensitizing pathways in the mature and immature ductus arteriosus
Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2007; 293(4): R1650 - R1656.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
M. Costa, S. Barogi, N. D. Socci, D. Angeloni, M. Maffei, B. Baragatti, C. Chiellini, E. Grasso, and F. Coceani
Gene expression in ductus arteriosus and aorta: comparison of birth and oxygen effects
Physiol Genomics, April 13, 2006; 25(2): 250 - 262.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
A. Bouayad, J.-C. Fouron, X. Hou, M. Beauchamp, C. Quiniou, D. Abran, K. Peri, R. I. Clyman, D. R. Varma, and S. Chemtob
Developmental regulation of prostaglandin E2 synthase in porcine ductus arteriosus
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2004; 286(5): R903 - R909.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
D. H. Wright, D. Abran, M. Bhattacharya, X. Hou, S. G. Bernier, A. Bouayad, J.-C. Fouron, A. Vazquez-Tello, M. H. Beauchamp, R. I. Clyman, et al.
Prostanoid receptors: ontogeny and implications in vascular physiology
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2001; 281(5): R1343 - R1360.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online