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Am J Physiol Heart Circ Physiol 280: H2462-H2469, 2001;
0363-6135/01 $5.00
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Vol. 280, Issue 6, H2462-H2469, June 2001

SPECIAL TOPIC
EDHF mediates flow-induced dilation in skeletal muscle arterioles of female eNOS-KO mice

An Huang1, Dong Sun1, Mairead A. Carroll2, Houli Jiang2, Carolyn J. Smith3, Joseph A. Connetta3, John R. Falck4, Edward G. Shesely5, Akos Koller1, and Gabor Kaley1

Departments of 1 Physiology, 2 Pharmacology, and 3 Pathology, New York Medical College, Valhalla, New York 10595; 4 Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas 75235; and 5 Division of Hypertension and Vascular Research, Henry Ford Hospital, Detroit, Michigan 48202

Vasodilation to increases in flow was studied in isolated gracilis muscle arterioles of female endothelial nitric oxide synthase (eNOS)-knockout (KO) and female wild-type (WT) mice. Dilation to flow (0-10 µl/min) was similar in the two groups, yet calculated wall shear stress was significantly greater in arterioles of eNOS-KO than in arterioles of WT mice. Indomethacin, which inhibited flow-induced dilation in vessels of WT mice by ~40%, did not affect the responses of eNOS-KO mice, whereas miconazole and 6-(2-proparglyoxyphenyl)hexanoic acid (PPOH) abolished the responses. Basal release of epoxyeicosatrienonic acids from arterioles was inhibited by PPOH. Iberiotoxin eliminated flow-induced dilation in arterioles of eNOS-KO mice but had no effect on arterioles of WT mice. In WT mice, neither Nomega -nitro-L-arginine methyl ester nor miconazole alone affected flow-induced dilation. Combination of both inhibitors inhibited the responses by ~50%. 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) alone inhibited flow-induced dilation by ~49%. ODQ + indomethacin eliminated the responses. Thus, in arterioles of female WT mice, nitric oxide and prostaglandins mediate flow-induced dilation. When eNOS is inhibited, endothelium-derived hyperpolarizing factor substitutes for nitric oxide. In female eNOS-KO mice, metabolites of cytochrome P-450, via activation of large-conductance Ca2+-activated K+ channels of smooth muscle, mediate entirely the arteriolar dilation to flow.

nitric oxide; prostaglandins; hyperpolarizing factor; cytochrome P-450 metabolites; potassium channels


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