| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Neurophysiology and 2 Department of Medicine III, University of Cologne, D-50931 Cologne, Germany
In embryonic stem (ES) cell-derived cardiomyocytes, spontaneous Ca2+ sparks representing Ca2+ release through ryanodine receptor (RyR) channels were characterized and correlated to the expression of RyRs as well as the Ca2+ load of the sarcoplasmic reticulum (SR). In very early developmental stage (VEDS) cardiac precursor cells, global intracellular Ca2+ concentration ([Ca2+]i) fluctuations occurred, whereas Ca2+ sparks and contractions were absent. In early developmental stages (EDS), contractions as well as Ca2+ sparks were obvious. During the further differentiation to late developmental stage (LDS) cardiomyocytes, a marked increase in the frequency of global [Ca2+]i transients, the amplitude and the frequency of Ca2+ sparks, as well as the expression of RyRs and the volume of RyR-positive SR, was observed. Furthermore, the caffeine-releasable SR Ca2+ load was elevated in LDS compared with EDS cardiomyocytes. A high-Ca2+ solution raised spark frequency as well as amplitude in EDS cardiomyocytes to the levels of LDS cardiomyocytes. The characteristics of Ca2+ sparks occurring in cardiomyocytes differentiated from ES cells may be governed by the Ca2+ load of the SR and/or the density of RyRs.
calcium-induced calcium release; embryoid body; ryanodine receptor
This article has been cited by other articles:
|
|
 |
|
  J. Satin, I. Itzhaki, S. Rapoport, E. A. Schroder, L. Izu, G. Arbel, R. Beyar, C. W. Balke, J. Schiller, and L. Gepstein Calcium Handling in Human Embryonic Stem Cell-Derived Cardiomyocytes Stem Cells, August 1, 2008; 26(8): 1961 - 1972. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Liu, J. D. Fu, C. W. Siu, and R. A. Li Functional Sarcoplasmic Reticulum for Calcium Handling of Human Embryonic Stem Cell-Derived Cardiomyocytes: Insights for Driven Maturation Stem Cells, December 1, 2007; 25(12): 3038 - 3044. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Kapur and K. Banach Inositol-1,4,5-trisphosphate-mediated spontaneous activity in mouse embryonic stem cell-derived cardiomyocytes J. Physiol., June 15, 2007; 581(3): 1113 - 1127. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Guan, S. Wagner, B. Unsold, L. S. Maier, D. Kaiser, B. Hemmerlein, K. Nayernia, W. Engel, and G. Hasenfuss Generation of Functional Cardiomyocytes From Adult Mouse Spermatogonial Stem Cells Circ. Res., June 8, 2007; 100(11): 1615 - 1625. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. White and W. C. Claycomb Embryonic stem cells form an organized, functional cardiac conduction system in vitro Am J Physiol Heart Circ Physiol, February 1, 2005; 288(2): H670 - H679. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Xu, M. Wani, Y.-S. Dai, J. Wang, M. Yan, A. Ayub, and M. Ashraf Differentiation of Bone Marrow Stromal Cells Into the Cardiac Phenotype Requires Intercellular Communication With Myocytes Circulation, October 26, 2004; 110(17): 2658 - 2665. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Reffelmann and R. A. Kloner Cellular cardiomyoplasty--cardiomyocytes, skeletal myoblasts, or stem cells for regenerating myocardium and treatment of heart failure? Cardiovasc Res, May 1, 2003; 58(2): 358 - 368. [Full Text] [PDF]
|
|
|
|
|
|
C. Perez-Terzic, A. Behfar, A. Mery, J. M.A. van Deursen, A. Terzic, and M. Puceat Structural Adaptation of the Nuclear Pore Complex in Stem Cell-Derived Cardiomyocytes Circ. Res., March 7, 2003; 92(4): 444 - 452. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
