| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Physiology, School of Medicine, Ehime University, Shigenobu, Onsen-gun, Ehime 791-0295, Japan
The effects of erythrocyte aggregation on O2 release were examined using O2-permeable fluorinated ethylenepropylene copolymer tubes (inner diameter, 25 µm; outer diameter, 100 µm). Measurements were performed using an apparatus built on an inverted microscope that contained a scanning-grating spectrophotometer with a photon count detector connected to two photomultipliers and an image processor through a video camera. The rate of O2 release from the cells flowing in the narrow tube was determined based on the visible absorption spectrum and the flow velocity of the cells as well as the tube size. When the tube was exposed to nitrogen-saturated deoxygenated saline containing 10 mM sodium dithionite, the flowing erythrocytes were deoxygenated in proportion to the traveling distance, and the deoxygenation at a given distance increased with decreasing flow velocity and cell concentration (hematocrit). Adding Dextran T-70 to the cell suspension increased erythrocyte aggregation in the tube, which resulted in suppressed cell deoxygenation and increased marginal cell-free-layer thickness. The deoxygenation was inversely proportional to the cell-free-layer thickness. The relation was not essentially altered even when the medium viscosity was adjusted with Dextran T-40 to remain constant. The rate of O2 release from erythrocytes in the tube was discussed in relation to the O2 diffusion process. We conclude that the diffusion of O2 from erythrocytes flowing in narrow tubes is inhibited primarily by erythrocyte aggregation itself and partly by thickening of the cell-free layer.
diffusion; cell-free layer; spectrophotometry; dextran; image processing
This article has been cited by other articles:
|
|
 |
|
  S. Kim, R. L. Kong, A. S. Popel, M. Intaglietta, and P. C. Johnson Temporal and spatial variations of cell-free layer width in arterioles Am J Physiol Heart Circ Physiol, September 1, 2007; 293(3): H1526 - H1535. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Levin, R. Gamzu, Y. Hasson, J.B. Lessing, A. Amit, I. Shapira, S. Berliner, and B. Almog Increased erythrocyte aggregation in ovarian hyperstimulation syndrome: a possible contributing factor in the pathophysiology of this disease Hum. Reprod., May 1, 2004; 19(5): 1076 - 1080. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D Zeltser, O Rogowski, S Berliner, T Mardi, D Justo, J Serov, M Rozenblat, D Avitzour, and I Shapira Sex differences in the expression of haemorheological determinants in individuals with atherothrombotic risk factors and in apparently healthy people Heart, March 1, 2004; 90(3): 277 - 281. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Sakai, Y. Suzuki, M. Kinoshita, S. Takeoka, N. Maeda, and E. Tsuchida O2 release from Hb vesicles evaluated using an artificial, narrow O2-permeable tube: comparison with RBCs and acellular Hbs Am J Physiol Heart Circ Physiol, December 1, 2003; 285(6): H2543 - H2551. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Ben-Ami, G. Barshtein, T. Mardi, V. Deutch, O. Elkayam, S. Yedgar, and S. Berliner A synergistic effect of albumin and fibrinogen on immunoglobulin-induced red blood cell aggregation Am J Physiol Heart Circ Physiol, December 1, 2003; 285(6): H2663 - H2669. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Cicha, Y. Suzuki, N. Tateishi, and N. Maeda Changes of RBC aggregation in oxygenation-deoxygenation: pH dependency and cell morphology Am J Physiol Heart Circ Physiol, June 1, 2003; 284(6): H2335 - H2342. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
