AJP - Heart Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 281: H661-H668, 2001;
0363-6135/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (13)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Detmer, K.
Right arrow Articles by Newman, W. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Detmer, K.
Right arrow Articles by Newman, W. H.
Vol. 281, Issue 2, H661-H668, August 2001

Endotoxin stimulated cytokine production in rat vascular smooth muscle cells

Kristina Detmer1, Zhongbiao Wang1, Debra Warejcka2, Sandra K. Leeper-Woodford1, and Walter H. Newman1,2

1 Division of Basic Medical Sciences and 2 Department of Surgery, Mercer University School of Medicine, Macon, Georgia 31207

Because inflammatory processes may promote the development of atherosclerosis, we examined the activation of cytokine genes in rat vascular smooth muscle cells in vitro after treatment with bacterial lipopolysaccharide (LPS). Interleukin-1 (IL-1), IL-6 and tumor necrosis factor-alpha (TNF-alpha ) mRNA increased in response to LPS. Activation of nuclear factor-kappa B (NF-kappa B) presumably results in NF-kappa B binding to regulatory regions of target genes and activating transcription. We therefore compared the kinetics of NF-kappa B activation, cytokine message production, and TNF-alpha secretion. Maximum active NF-kappa B was found at 30 min after the addition of LPS and decreased thereafter. Increased IL-6 mRNA was detected at 30 min, increased TNF-alpha mRNA at 60 min, and increased IL-1 mRNA at 120 min. Secretion of TNF-alpha was dependent on LPS concentration and was first detected 120 min after LPS addition. Aspirin, which has been shown to inhibit NF-kappa B activation and cytokine secretion in other cell types, did not inhibit NF-kappa B activation or TNF-alpha secretion. However, aspirin reduced the amount of both TNF-alpha and IL-6 mRNA present 30 min after LPS addition by half (P < 0.05).

atherosclerosis; nuclear factor-kappa B; tumor necrosis factor-alpha ; aspirin; cytokine gene transcription


This article has been cited by other articles:


Home page
 J. Immunol.Home page
J. S. Hui-Yuen, T. T. Duong, and R. S. M. Yeung
TNF-{alpha} Is Necessary for Induction of Coronary Artery Inflammation and Aneurysm Formation in an Animal Model of Kawasaki Disease
J. Immunol., May 15, 2006; 176(10): 6294 - 6301.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
S. Banzet, N. Koulmann, N. Simler, O. Birot, H. Sanchez, R. Chapot, A. Peinnequin, and X. Bigard
Fibre-type specificity of interleukin-6 gene transcription during muscle contraction in rat: association with calcineurin activity
J. Physiol., August 1, 2005; 566(3): 839 - 847.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
W. Li, K. Tanaka, A. Ihaya, Y. Fujibayashi, S. Takamatsu, K. Morioka, M. Sasaki, T. Uesaka, T. Kimura, N. Yamada, et al.
Gene therapy for chronic myocardial ischemia using platelet-derived endothelial cell growth factor in dogs
Am J Physiol Heart Circ Physiol, January 1, 2005; 288(1): H408 - H415.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online