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Am J Physiol Heart Circ Physiol 281: H755-H763, 2001;
0363-6135/01 $5.00
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Vol. 281, Issue 2, H755-H763, August 2001

Hypertonic saline method accurately determines parallel conductance for dual-field conductance catheter

Paul Steendijk, Eva Staal, J. Wouter Jukema, and Jan Baan

Department of Cardiology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands

Conversion of conductance catheter data to absolute ventricular volumes requires assessment of parallel conductance (GP). We determined the accuracy of GP obtained by the hypertonic saline method (G<UP><SUB>saline</SUB><SUP>P</SUP></UP>) compared with angiographically derived GP (G<UP><SUB>Angio</SUB><SUP>P</SUP></UP>) and quantified the variabilities of GP for the dual-field conductance catheter method in nine anesthetized sheep studied at baseline, treated with dobutamine, and subjected to volume loading and beta -blockade. G<UP><SUB>saline</SUB><SUP>P</SUP></UP> and G<UP><SUB>Angio</SUB><SUP>P</SUP></UP> showed an excellent linear correlation (G<UP><SUB>saline</SUB><SUP>P</SUP></UP> = 1.002 · G<UP><SUB>Angio</SUB><SUP>P</SUP></UP> + 0.001 Omega -1, R2 = 0.92), and Bland-Altman analysis yielded a nonsignificant bias and narrow limits of agreement (bias ± 2SD = 0.002 ± 0.112 Omega -1). Within-animal variability of GP was very similar with both methods and was due to changes in blood conductivity rather than geometrical changes. Variability between animals was significant (26.3% of mean for G<UP><SUB>saline</SUB><SUP>P</SUP></UP> and 25.7% for G<UP><SUB>Angio</SUB><SUP>P</SUP></UP>) and thus warrants individual assessment. Variations during the cardiac cycle were not significantly different from zero. With biplane angiography used as gold standard, the hypertonic saline method accurately determines GP for the dual-field conductance catheter over a wide range of hemodynamic conditions.

angiography; left ventricular volume; sheep; ventricular function


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