AJP - Heart AJP: Lung Cellular and Molecular Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 281: H873-H881, 2001;
0363-6135/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (16)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tuttle, J. L.
Right arrow Articles by Falcone, J. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tuttle, J. L.
Right arrow Articles by Falcone, J. C.
Vol. 281, Issue 2, H873-H881, August 2001

Nitric oxide release during alpha 1-adrenoceptor-mediated constriction of arterioles

Jay L. Tuttle and Jeff C. Falcone

Department of Physiology and Biophysics, University of Louisville School of Medicine, Louisville, Kentucky 40292

We examined endothelial modulation of norepinephrine (NE)-mediated constriction in isolated, cannulated, first-order arterioles from skeletal muscle of rats. Acute arteriolar constrictor responses to NE (10-9 to 10-7 M) were significantly (P < 0.05) enhanced after either endothelial denudation or inhibition of nitric oxide synthase with NG-monomethyl-L-arginine (10-4 M, 30 min). In contrast, arteriolar constrictions to NE were not different after treatment with either the cyclooxygenase inhibitor diclofenac (10-6 M, 30 min) or the K+-channel blocker tetrabutylammonium (5 × 10-5 M, 30 min). We also measured arteriolar responses to the vasoconstrictor PGF2alpha ; responses were not altered by any of the experimental treatments, which indicates that this phenomenon is not ubiquitous to all vasoconstricting agents. Mechanistically, we examined vascular smooth muscle (VSM) and endothelial cell calcium. Both NE and PGF2alpha significantly increased VSM cell calcium measurements; however, endothelial cell calcium was significantly increased with NE or phenylephrine (an alpha 1-adrenergic agonist) but not with PGF2alpha or UK-14304 (an alpha 2-adrenergic agonist). Together these findings suggest that in rat cremaster first-order arterioles, NE stimulates an increase in VSM calcium via adrenergic receptors with subsequent increase in endothelial cell calcium, possibly via stimulation of alpha 1-adrenergic receptors on the arteriolar endothelium. The burst in endothelial cell calcium may then lead to the production of nitric oxide, which diffuses to the VSM, attenuates constriction, and maintains at least some minimal level of blood flow.

norepinephrine; vascular smooth muscle; endothelial cell calcium; protstglandin F2alpha ; phenylephrine; UK-14304


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. C. Vinci, L. Bellik, S. Filippi, F. Ledda, and A. Parenti
Trophic effects induced by {alpha}1D-adrenoceptors on endothelial cells are potentiated by hypoxia
Am J Physiol Heart Circ Physiol, October 1, 2007; 293(4): H2140 - H2147.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. F. Brekke, W. F. Jackson, and S. S. Segal
Arteriolar smooth muscle Ca2+ dynamics during blood flow control in hamster cheek pouch
J Appl Physiol, July 1, 2006; 101(1): 307 - 315.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
A. P. McKee, D. A. Van Riper, C. A. Davison, and H. A. Singer
Gender-dependent modulation of alpha 1-adrenergic responses in rat mesenteric arteries
Am J Physiol Heart Circ Physiol, May 1, 2003; 284(5): H1737 - H1743.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. Okajima, M. Takamura, P. Vequaud, R. Parent, and M. Lavallee
beta -Adrenergic receptor blockade impairs NO-dependent dilation of large coronary arteries during exercise
Am J Physiol Heart Circ Physiol, February 1, 2003; 284(2): H501 - H510.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online