AJP - Heart Calcium Transients and Cell-Sarcomere
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Am J Physiol Heart Circ Physiol 281: H969-H974, 2001;
0363-6135/01 $5.00
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Vol. 281, Issue 2, H969-H974, August 2001

SPECIAL COMMUNICATION
High-throughput assessment of calcium sensitivity in skinned cardiac myocytes

Chee Chew Lim*, Michiel H. B. Helmes*, Douglas B. Sawyer, Mohit Jain, and Ronglih Liao

Whitaker Cardiovascular Institute and Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118

Isolated permeabilized cardiac myocytes have been used in the study of myofilament calcium sensitivity through measurement of the isometric force-pCa curve. Determining this force-pCa relationship in skinned myocytes is relatively expensive and carries a high degree of variability. We therefore attempted to establish an alternative high-throughput method to measure calcium sensitivity in cardiac myocytes. With the use of commercially available software that allows for precise measurement of sarcomere spacing, we measured sarcomere length changes in unloaded skinned cardiac myocytes over a range of calcium concentrations. With the use of this technique, we were able to accurately detect acute increases or decreases in myofilament calcium sensitivity after exposure to 10 mM caffeine or 5 mM 2,3-butanedione monoxime, respectively. This technique allows for the simple and rapid determination of myofilament calcium sensitivity in cardiac myocytes in a reproducible and inexpensive manner and could be used for high-throughput screening of pharmacological agents and/or transgenic mouse models for changes in myofilament calcium sensitivity.

myofilament; sarcomere length

* Authors contributed equally to this study.




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