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Am J Physiol Heart Circ Physiol 281: H1372-H1379, 2001;
0363-6135/01 $5.00
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Vol. 281, Issue 3, H1372-H1379, September 2001

Existence of cardiac PNMT mRNA in adult rats: elevation by stress in a glucocorticoid-dependent manner

O. Krizanová1,2, L. Micutková1, J. Jeloková1, M. Filipenko3, E. Sabban4, and R. Kvetnanský1

1 Institute of Experimental Endocrinology and 2 Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, 833 34 Bratislava, Slovak Republic; 3 Institute of Bioorganic Chemistry, Russian Academy of Sciences, Novosibirsk 630090, Russia; and 4 Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595

Phenylethanolamine N-methyltransferase (PNMT) is the enzyme that synthesizes epinephrine from norepinephrine. The aim of this study was to determine potential PNMT gene expression in the cardiac atria and ventricles of adult rats and to examine whether the gene expression of this enzyme is affected by immobilization stress. PNMT mRNA levels were detected in all four parts of the heart, with the highest level in the left atrium. Both Southern blot and sequencing verified the specificity of PNMT detected by RT-PCR. Single immobilization for 2 h increased gene expression of PNMT in both atria and ventricles. In atria, this effect was clearly modulated by glucocorticoids, because either adrenalectomy or hypophysectomy prevented the increase in PNMT mRNA levels in response to immobilization stimulus. This study establishes, for the first time, that PNMT gene expression occurs in cardiac atria and also, to a small extent, in ventricles of adult rats. Immobilization stress increases gene expression in atria and ventricles. This increase requires an intact hypothalamus-pituitary-adrenocortical axis, indicating the involvement of glucocorticoids.

effect of glucocorticoids; adrenalectomy; hypophysectomy; phenylethanolamine N-methyltransferase


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