AJP - Heart AJP: Renal Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 281: H1397-H1406, 2001;
0363-6135/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (24)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tyml, K.
Right arrow Articles by Ouellette, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tyml, K.
Right arrow Articles by Ouellette, Y.
Vol. 281, Issue 3, H1397-H1406, September 2001

Lipopolysaccharide reduces intercellular coupling in vitro and arteriolar conducted response in vivo

Karel Tyml1,3, Xiaowei Wang1,3, Darcy Lidington2,3, and Yves Ouellette2

1 A. C. Burton Laboratory and 2 Child Health Research Institute, Lawson Health Research Institute, and 3 Department of Medical Biophysics, University of Western Ontario, London, Ontario, Canada N6A 5C1

Our recent in vitro study (Lidington et al. J Cell Physiol 185: 117-125, 2000) suggested that lipopolysaccharide (LPS) reduces communication along blood vessels. The present investigation extended this study to determine whether any effect of LPS and/or inflammatory cytokines [tumor necrosis factor-alpha , interleukin (IL)-1beta , and IL-6] on endothelial cell coupling in vitro could also be demonstrated for an arteriolar conducted response in vivo. Using an electrophysiological approach in monolayers of microvascular endothelial cells, we found that LPS (10 µg/ml) but not these cytokines reduced intercellular conductance (ci) (an index of cell communication) and that LPS together with these cytokines did not further reduce ci. Also, ci was restored after LPS washout, and the LPS-induced reduction was prevented by protein tyrosine kinase (PTK) inhibitors (1.5 µM Tyr A9 and 10 nM PP-2). In our in vivo experiments in arterioles of the mouse cremaster muscle, local electrical stimulation evoked vasoconstriction that conducted along arterioles. LPS in the muscle superfusate did not alter local vasoconstriction but reduced the conducted response. Washout of LPS restored the conducted response, whereas PTK inhibitors prevented the effect of LPS. On the basis of a newly developed mathematical model, the LPS-induced reduction in conducted response was predicted to reduce the arteriolar ability to increase resistance to blood flow. We conclude that LPS can reduce communication in in vitro and in vivo systems comparably in a reversible and tyrosine kinase-dependent manner. Based on literature and present results, we suggest that LPS may compromise microvascular hemodynamics at both the arteriolar responsiveness and the conduction levels.

endothelial cell monolayer; mouse cremaster muscle; tyrosine kinase; mathematical model


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
B. Rodenwaldt, U. Pohl, and C. de Wit
Endogenous and exogenous NO attenuates conduction of vasoconstrictions along arterioles in the microcirculation
Am J Physiol Heart Circ Physiol, May 1, 2007; 292(5): H2341 - H2348.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
C. de Wit
Closing the Gap at Hot Spots
Circ. Res., April 13, 2007; 100(7): 931 - 933.
[Full Text] [PDF]

Home page
 Cardiovasc ResHome page
R. L. McKinnon, D. Lidington, M. Bolon, Y. Ouellette, G. M. Kidder, and K. Tyml
Reduced arteriolar conducted vasoconstriction in septic mouse cremaster muscle is mediated by nNOS-derived NO
Cardiovasc Res, January 1, 2006; 69(1): 236 - 244.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
K. Tyml, D. Anderson, D. Lidington, and H. M. Ladak
A new method for assessing arteriolar diameter and hemodynamic resistance using image analysis of vessel lumen
Am J Physiol Heart Circ Physiol, May 1, 2003; 284(5): H1721 - H1728.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online