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1 Departments of Neurosurgery, 2 Pathology, and 3 Physiology, University of Maryland School of Medicine, Baltimore, Maryland 21201
Cells in blood vessel walls express connexin (Cx)43,
Cx40, and Cx37. We recently characterized gap junction channels in rat basilar artery smooth muscle cells and found features attributable not
only to these three connexins but also to an unidentified connexin,
including strong voltage dependence and single channel conductance of
30-40 pS. Here, we report data consistent with identification of
Cx45. Immunofluorescence using anti-human Cx45 and anti-mouse Cx45
antibodies revealed labeling between 
-actin-positive cells, and
RT-PCR of mRNA from arteries after endothelial destruction yielded
amplicons exhibiting 90-98% identity with mouse Cx45 and human
Cx45. Dual-perforated patch clamping was performed after exposure to
oligopeptides that interfere with docking of Cx43, Cx40, or Cx45. Cell
pairs pretreated with blocking peptides for Cx43 and Cx40 exhibited
strongly voltage-dependent transjunctional conductances [voltage at
which voltage-dependent conductance declines by one-half
(V1/2) = ±18.9 mV] and small single
channel conductances (31 pS), consistent with the presence of Cx45,
whereas cell pairs pretreated with blocking peptide for Cx45 exhibit
weaker voltage-dependent conductances (V1/2 = ±37.9 mV), consistent with block of Cx45. Our data suggest that Cx45
is transcribed, expressed, and forms functional gap junction channels
in rat cerebral arterial smooth muscle.
gap junctions; vascular smooth muscle; patch clamp
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