| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Laboratory of Oxygen Metabolism and 2 Nuclear Medicine Center, University Hospital, University of Buenos Aires, 1120 Buenos Aires, Argentina
Changes
in O2 uptake at different thyroid status have been
explained on the basis of the modulation of mitochondrial enzymes and
membrane biophysical properties. Regarding the nitric oxide (NO)
effects, we tested whether liver mitochondrial nitric oxide synthase
(mtNOS) participates in the modulation of O2 uptake in thyroid disorders. Wistar rats were inoculated with 400 µCi
131I (hypothyroid group), 20 µg thyroxine
(T4)/100 g body wt administered daily for 2 wk
(hyperthyroid group) or vehicle (control). Basal metabolic rate,
mitochondrial function, and mtNOS activity were analyzed. Systemic and
liver mitochondrial O2 uptake and cytochrome oxidase
activity were lower in hypothyroid rats with respect to controls;
mitochondrial parameters were further decreased by
L-arginine (
42 and 34%, P < 0.05),
consistent with 5- to 10-fold increases in matrix NO concentration.
Accordingly, mtNOS expression (75%) and activity (260%) were
selectively increased in hypothyroidism and reverted by hormone
replacement without changes in other nitric oxide isoforms. Moreover,
mtNOS activity correlated with serum 3,5,3'-triiodothyronine
(T3) and O2 uptake. Increased mtNOS activity was also observed in skeletal muscle mitochondria from hypothyroid rats. Therefore, we suggest that modulation of mtNOS is a substantial part of thyroid effects on mitochondrial O2 uptake.
hypothyroidism; oxygen uptake regulation; hyperthyroidism
This article has been cited by other articles:
|
|
 |
|
  M. C. Carreras and J. J. Poderoso Mitochondrial nitric oxide in the signaling of cell integrated responses Am J Physiol Cell Physiol, May 1, 2007; 292(5): C1569 - C1580. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Fellet, A. M. Balaszczuk, C. Arranz, J. J. Lopez-Costa, A. Boveris, and J. Bustamante Autonomic regulation of pacemaker activity: role of heart nitric oxide synthases Am J Physiol Heart Circ Physiol, September 1, 2006; 291(3): H1246 - H1254. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Zaobornyj, L. B. Valdez, P. La Padula, L. E. Costa, and A. Boveris Effect of sustained hypobaric hypoxia during maturation and aging on rat myocardium. II. mtNOS activity J Appl Physiol, June 1, 2005; 98(6): 2370 - 2375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. F. Gonzales, F. A. Chung, S. Miranda, L. B. Valdez, T. Zaobornyj, J. Bustamante, and A. Boveris Heart mitochondrial nitric oxide synthase is upregulated in male rats exposed to high altitude (4,340 m) Am J Physiol Heart Circ Physiol, June 1, 2005; 288(6): H2568 - H2573. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Fellet, P. Arza, N. Arreche, C. Arranz, and A. M. Balaszczuk Nitric oxide and thyroid gland: modulation of cardiovascular function in autonomic-blocked anaesthetized rats Exp Physiol, May 1, 2004; 89(3): 303 - 312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Zaninovich, I. Rebagliati, M. Raices, C. Ricci, and K. Hagmuller Mitochondrial respiration in muscle and liver from cold-acclimated hypothyroid rats J Appl Physiol, October 1, 2003; 95(4): 1584 - 1590. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Galli, M. I. Labato, E. Bal de Kier Joffe, M. C. Carreras, and J. J. Poderoso Decreased Mitochondrial Nitric Oxide Synthase Activity and Hydrogen Peroxide Relate Persistent Tumoral Proliferation to Embryonic Behavior Cancer Res., October 1, 2003; 63(19): 6370 - 6377. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Harper, A. T. Galecki, D. T. Burke, S. L. Pinkosky, and R. A. Miller Quantitative trait loci for insulin-like growth factor I, leptin, thyroxine, and corticosterone in genetically heterogeneous mice Physiol Genomics, September 29, 2003; 15(1): 44 - 51. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Peralta, P. V. Finocchietto, D. Converso, F. Schopfer, M. C. Carreras, and J. J. Poderoso Modulation of mitochondrial nitric oxide synthase and energy expenditure in rats during cold acclimation Am J Physiol Heart Circ Physiol, June 1, 2003; 284(6): H2375 - H2383. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. A. Riobo, M. Melani, N. Sanjuan, M. L. Fiszman, M. C. Gravielle, M. C. Carreras, E. Cadenas, and J. J. Poderoso The Modulation of Mitochondrial Nitric-oxide Synthase Activity in Rat Brain Development J. Biol. Chem., November 1, 2002; 277(45): 42447 - 42455. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Zaninovich, M. Raices, I. Rebagliati, C. Ricci, and K. Hagmuller Brown fat thermogenesis in cold-acclimated rats is not abolished by the suppression of thyroid function Am J Physiol Endocrinol Metab, September 1, 2002; 283(3): E496 - E502. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. H. Hintze Prologue: Nitric oxide-hormones, metabolism, and function Am J Physiol Heart Circ Physiol, December 1, 2001; 281(6): H2253 - H2255. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
