| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 University Laboratory of Physiology, Oxford OX1 3PT; 2 Department of Human Anatomy and Genetics, Oxford OX1 3QX, United Kingdom; and 3 Department of Physiology, Monash University, Victoria 3800, Australia
The role of nitric oxide (NO) in the vagal
control of heart rate (HR) is controversial. We investigated the
cholinergic regulation of HR in isolated atrial preparations with an
intact right vagus nerve from wild-type (nNOS+/+, n = 81) and neuronal NO synthase (nNOS) knockout (nNOS
/,
n = 43) mice. nNOS was immunofluorescently colocalized
within choline-acetyltransferase-positive neurons in nNOS+/+ atria. The
rate of decline in HR during vagal nerve stimulation (VNS, 3 and 5 Hz)
was slower in nNOS/ compared with nNOS+/+ atria in vitro
(P < 0.01). There was no difference between the HR
responses to carbamylcholine in nNOS+/+ and nNOS/ atria. Selective
nNOS inhibitors, vinyl-L-niohydrochloride or
1-2-trifluoromethylphenyl imidazole, or the guanylyl cyclase
inhibitor, 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one significantly (P < 0.05) attenuated the decrease in HR
with VNS at 3 Hz in nNOS+/+ atria. NOS inhibition had no effect in
nNOS/ atria during VNS. In all atria, the NO donor sodium
nitroprusside significantly enhanced the magnitude of the vagal-induced
bradycardia, showing the downstream intracellular pathways activated by
NO were intact. These results suggest that neuronal NO facilitates vagally induced bradycardia via a presynaptic modulation of neurotransmission.
nitric oxide; parasympathetic; sinoatrial node
This article has been cited by other articles:
|
|
 |
|
  J. K. Choate, S. M. Murphy, R. Feldman, and C. R. Anderson Sympathetic control of heart rate in nNOS knockout mice Am J Physiol Heart Circ Physiol, January 1, 2008; 294(1): H354 - H361. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. D. Wadley, J. Choate, and G. K. McConell NOS isoform-specific regulation of basal but not exercise-induced mitochondrial biogenesis in mouse skeletal muscle J. Physiol., November 15, 2007; 585(1): 253 - 262. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Heaton, D. Li, S. C. Almond, T. A. Dawson, L. Wang, K. M. Channon, and D. J. Paterson Gene Transfer of Neuronal Nitric Oxide Synthase into Intracardiac Ganglia Reverses Vagal Impairment in Hypertensive Rats Hypertension, February 1, 2007; 49(2): 380 - 388. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Casadei The emerging role of neuronal nitric oxide synthase in the regulation of myocardial function Exp Physiol, November 1, 2006; 91(6): 943 - 955. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Cook, M. Togni, M. C. Schaub, P. Wenaweser, and O. M. Hess High heart rate: a cardiovascular risk factor? Eur. Heart J., October 2, 2006; 27(20): 2387 - 2393. [Full Text] [PDF]
|
|
|
|
 |
|
 M. Wehling-Henricks, M. C. Jordan, K. P. Roos, B. Deng, and J. G. Tidball Cardiomyopathy in dystrophin-deficient hearts is prevented by expression of a neuronal nitric oxide synthase transgene in the myocardium Hum. Mol. Genet., July 15, 2005; 14(14): 1921 - 1933. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. J. DANSON and D. J. PATERSON Cardiac Neurobiology of Nitric Oxide Synthases Ann. N.Y. Acad. Sci., June 1, 2005; 1047(1): 183 - 196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Farias III, K. Jackson, M. Johnson, and J. L. Caffrey Cardiac enkephalins attenuate vagal bradycardia: interactions with NOS-1-cGMP systems in canine sinoatrial node Am J Physiol Heart Circ Physiol, November 1, 2003; 285(5): H2001 - H2012. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P.B. Massion, O. Feron, C. Dessy, and J.-L. Balligand Nitric Oxide and Cardiac Function: Ten Years After, and Continuing Circ. Res., September 5, 2003; 93(5): 388 - 398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Choate and R. Feldman Neuronal control of heart rate in isolated mouse atria Am J Physiol Heart Circ Physiol, August 7, 2003; 285(3): H1340 - H1346. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Ortiz and J. L. Garvin Cardiovascular and renal control in NOS-deficient mouse models Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2003; 284(3): R628 - R638. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R.M. Mohan, D.A. Heaton, E.J.F. Danson, S.P.R. Krishnan, S. Cai, K.M. Channon, and D.J. Paterson Neuronal Nitric Oxide Synthase Gene Transfer Promotes Cardiac Vagal Gain of Function Circ. Res., December 13, 2002; 91(12): 1089 - 1091. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Herring, E. J. F. Danson, and D. J. Paterson Cholinergic Control of Heart Rate by Nitric Oxide is Site Specific Physiology, October 1, 2002; 17(5): 202 - 206. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Herring, D. J. Paterson, F. Brunner, P. Andrew, G. Woslkart, B. Mayer, and R. Zechner Endothelial Nitric Oxide Synthase and Heart Rate * Response Circulation, July 9, 2002; 106 (2): e5 - e5. [Full Text] [PDF]
|
|
|
|
|
|
B. J. A. Janssen and J. F. M. Smits Autonomic control of blood pressure in mice: basic physiology and effects of genetic modification Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2002; 282(6): R1545 - R1564. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Herring, J. A.B. Zaman, and D. J. Paterson Particulate guanylyl cyclase and cholinergic control of cardiac excitability is site specific Cardiovasc Res, June 1, 2002; 54(3): 697 - 698. [Full Text] [PDF]
|
|
|
|
|
|
T. H. Hintze Prologue: Nitric oxide-hormones, metabolism, and function Am J Physiol Heart Circ Physiol, December 1, 2001; 281(6): H2253 - H2255. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
