AJP - Heart Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 281: H2654-H2660, 2001;
0363-6135/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (5)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hardin, C. D.
Right arrow Articles by Rovetto, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hardin, C. D.
Right arrow Articles by Rovetto, M. J.
Vol. 281, Issue 6, H2654-H2660, December 2001

Myocardial metabolism of exogenous FDP is consistent with transport by a dicarboxylate transporter

Christopher D. Hardin1, Giuseppe Lazzarino3, Barbara Tavazzi2, Donato Di Pierro2, Tina M. Roberts1, Bruno Giardina4, and Michael J. Rovetto1,5

1 Department of Physiology, University of Missouri, Columbia, 65212; 2 Department of Experimental Medicine and Biochemical Sciences, University of Rome Tor Vergata, Rome; 3 Department of Chemical Sciences, Laboratory of Biochemistry, University of Catania, Catania; 4 Institute of Chemistry and Centro per lo studio sulla chimica dei recettori, Catholic University Sacro Cuore, Rome 00168, Italy; and 5 Dalton Cardiovascular Research Center, University of Missouri, Columbia, Missouri 65212

The extent to and the mechanism by which fructose-1,6-bisphosphate (FDP) crosses cell membranes are unknown. We hypothesized that its transport is either via band 3 or a dicarboxylate transporter. The question was addressed in isolated Langendorff rat hearts perfused under normoxic conditions. Groups of hearts received the following metabolic substrates (in mM): 5 FDP; 5 FDP + either 5, 10, or 20 fumarate; 10 FDP and either 5, 10, or 20 fumarate; or 5 FDP + 2 4,4'-dinitrostilbene-2,2'-disulfonate (DNDS), a band 3 inhibitor. FDP uptake and metabolism were measured as production of [13C]lactate from [13C]FDP or 14CO2 and [14C]lactate from uniformly labeled [14C]FDP in sample perfusates. During 30 min of perfusion, FDP metabolism was 12.4 ± 2.6 and 31.2 ± 3.0 µmol for 5 and 10 mM FDP, respectively. Addition of 20 mM fumarate reduced FDP metabolism over a 30-min perfusion period to 3.1 ± 0.6 and 6.3 ± 0.5 µmol for 5 and 10 mM FDP groups, respectively. DNDS did not affect FDP utilization. These data are consistent with transport of FDP by a dicarboxylate transport system.

glycolysis; energetics; dicarboxylic acid metabolism; band 3 transporter; biological transport


This article has been cited by other articles:


Home page
 Anesth. Analg.Home page
B. J. Riedel, J. Gal, G. Ellis, P. J. Marangos, A. W. Fox, and D. Royston
Myocardial Protection Using Fructose-1,6-Diphosphate During Coronary Artery Bypass Graft Surgery: A Randomized, Placebo-Controlled Clinical Trial
Anesth. Analg., January 1, 2004; 98(1): 20 - 29.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online