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Am J Physiol Heart Circ Physiol 281: H2731-H2737, 2001;
0363-6135/01 $5.00
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Vol. 281, Issue 6, H2731-H2737, December 2001

Nutritive blood flow affects microdialysis O/I ratio for [14C]ethanol and 3H2O in perfused rat hindlimb

John M. B. Newman, Carla A. Di Maria, Stephen Rattigan, and Michael G. Clark

Department of Biochemistry, Medical School, University of Tasmania, Hobart 7001, Australia

Changes in the microdialysis outflow-to-inflow (O/I) ratio for [14C]ethanol and 3H2O were determined in the perfused rat hindlimb after increases and decreases in nutritive flow mediated by the vasoconstrictors norepinephrine (NE) and serotonin (5-HT), respectively. Microdialysis probes (containing 10 mM [14C]ethanol and 3H2O pumped at 1 or 2 µl/min) were inserted through the calf of the rat. Hindlimb perfusion flow rate was varied from 6 to 56 ml · min-1 · 100 g-1 in the presence of NE, 5-HT, or saline vehicle. The O/I ratios for both tracers were determined at each perfusion flow rate, as was perfusion pressure, oxygen uptake (a surrogate indicator of nutritive flow), and lactate release. Both tracers showed a decreased O/I ratio as hindlimb perfusion flow was increased, with [14C]ethanol being higher than 3H2O. NE decreased the O/I ratio compared with vehicle, and 5-HT increased it for both tracers and both microdialysis flow rates. We conclude that the microdialysis O/I ratio, while able to detect changes in total flow, is also sensitive to changes in nutritive and nonnutritive flow, where the latter still extracts tracer, but less than the former.

vasoconstrictors; interstitial fluid; nutritive/nonnutritive blood flow; total blood flow; skeletal muscle; outflow-to-inflow ratio


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