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Am J Physiol Heart Circ Physiol 281: H2757-H2761, 2001;
0363-6135/01 $5.00
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Vol. 281, Issue 6, H2757-H2761, December 2001

SPECIAL COMMUNICATION
Respiratory alkalosis does not alter NOx concentrations in human plasma and erythrocytes

Takaharu Ishibashi1, Kaname Kubota3, Mariko Himeno2, Taku Matsubara3, Tomoyuki Hori3, Kazuyuki Ozaki3, Masaru Yamozoe3, Yoshifusa Aizawa3, Junko Yoshida1, and Matomo Nishio1

1 Department of Pharmacology and 2 Division of Endocrinology, Department of Internal Medicine, Kanazawa Medical University, Uchinada, Ishikawa 920-0293; and 3 The First Department of Internal Medicine, Niigata University School of Medicine, Niigata, 951-8510 Japan

To test the hypothesis that NOx (NO<UP><SUB>2</SUB><SUP>−</SUP></UP> and NO<UP><SUB>3</SUB><SUP>−</SUP></UP>, metabolites of NO) accumulates in red blood cells (RBC) in response to changes in PCO2 and bicarbonate (HCO<UP><SUB>3</SUB><SUP>−</SUP></UP>) concentration in blood, we examined the effect of changes in PCO2 and HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> induced by hyperventilation in healthy adults on partitioning of NOx in whole blood. NOx in hemolysate was measured by a high-performance liquid chromatography-Griess system equipped with a C18 reverse phase column to trap hemoglobin, which enables determination of whole blood NOx concentration and calculation of NOx concentration in RBC with high accuracy and reproducibility. NOx concentration in RBC was lower than that in plasma, and equilibrium between plasma and RBC was achieved rapidly after addition of NO<UP><SUB>3</SUB><SUP>−</SUP></UP>. Changes in PCO2 and HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> by hyperventilation failed to influence NOx concentrations in both plasma and RBC. Plasma NOx concentrations correlated with whole blood NOx and RBC NOx concentrations. Our results indicate that changes in PCO2 or HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> induced by hyperventilation do not influence NOx compartmentalization in plasma and RBC.

nitrate; hemolysate; nitric oxide metabolite measurement; hyperventilation


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Malnutrition impairs alveolar fluid clearance in rat lungs
Am J Physiol Lung Cell Mol Physiol, June 1, 2004; 286(6): L1268 - L1274.
[Abstract] [Full Text] [PDF]


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