AJP - Heart Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 282: H414-H422, 2002. First published October 11, 2001; doi:10.1152/ajpheart.00292.2001
0363-6135/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/2/H414    most recent
00292.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (17)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Medhora, M.
Right arrow Articles by Jacobs, E. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Medhora, M.
Right arrow Articles by Jacobs, E. R.
Vol. 282, Issue 2, H414-H422, February 2002

Upregulation of collagens detected by gene array in a model of flow-induced pulmonary vascular remodeling

Meetha Medhora1, Michael Bousamra II3, Daling Zhu1, Lewis Somberg2, and Elizabeth R. Jacobs1

1 Department of Medicine and Cardiovascular Research Center and 2 Department of Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin 53226; and 3 Department of Surgery, University of Louisville, and Jewish Hospital Heart Institute, Louisville, Kentucky 40241

We recently reported localized increased pulmonary arterial resistance, neointimal lesions, and medial thickening induced by aortopulmonary anastomosis in young pigs. This model was used to investigate changes in expression of genes potentially involved in pulmonary vascular remodeling employing a high throughput Atlas Human Cardiovascular Array carrying ~600 cardiovascular-related cDNA sequences. Data were confirmed by Northern analysis, Western blots, and histological examination. With the use of lower stringency conditions for hybridization, 56% of the 588 human genes on the array showed visible signal after autoradiography. Approximately 10% of the genes with visible hybridization were altered by shunt-induced high flow. Extracellular matrix and cell adhesion molecules were the most highly represented group of upregulated genes. To our knowledge, our data are the first to demonstrate flow-induced changes in gene expression using a combination of cross species cDNA arrays, homologous hybridization, immunospecific protein, and histology. Our observations expand the list of genes as putative candidates in pulmonary vascular remodeling and support the utility of cross-species microarray analysis in such applications.

pulmonary hypertension; pulmonary arteries; shear; high flow; vasculopathy; cardiovascular; porcine


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
B. A. Simon, R. B. Easley, D. N. Grigoryev, S.-F. Ma, S. Q. Ye, T. Lavoie, R. M. Tuder, and J. G. N. Garcia
Microarray analysis of regional cellular responses to local mechanical stress in acute lung injury
Am J Physiol Lung Cell Mol Physiol, November 1, 2006; 291(5): L851 - L861.
[Abstract] [Full Text] [PDF]

Home page
 HeartHome page
C Napoli, L O Lerman, V Sica, A Lerman, G Tajana, and F de Nigris
Microarray analysis: a novel research tool for cardiovascular scientists and physicians
Heart, June 1, 2003; 89(6): 597 - 604.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online